To stain for α-SMA, paraffin-embedded liver sections were incubated with rabbit anti-α-SMA primary antibody (Abcam, 1:50) and visualized with Histostain Plus kit (Invitrogen).
Rabbit anti α sma primary antibody
Manufactured by Abcam
Sourced in United Kingdom
Rabbit anti-α-SMA primary antibody is a laboratory reagent used for the detection and quantification of alpha-smooth muscle actin (α-SMA) in various sample types. It is a polyclonal antibody raised in rabbits and is designed for use in techniques such as immunohistochemistry, western blotting, and ELISA.
Automatically generated - may contain errors
Lab products found in correlation
Histostain plus kit, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit secondary antibody conjugated to alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Alexa fluor dye, by Thermo Fisher Scientific (1 mentions)
Lsm 700 confocal laser scanning microscope, by Zeiss (1 mentions)
3 protocols using rabbit anti α sma primary antibody
1
Immunohistochemical Staining of α-SMA
2
Immunofluorescence Staining of α-SMA in mCFs
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Measurement of α-SMA using immunofluorescence staining was performed as previously described [12 (link)]. Briefly, after being placed in 24 well plates, mCFs were washed with PBS twice and then were blocked with 1 % bovine serum albumin at room temperature for 30 min. Then the cells were incubated overnight with rabbit anti-α-SMA primary antibody (1:100, Abcam). After being washed with PBS, the cells were performed with goat anti-rabbit secondary antibody conjugated to Alexa Fluor 488 (1:400, Invitrogen) for 1 h under dark condition. Finally, the cells were counterstained with DAPI (Sigma) for 10 min, and were analyzed with an immunofluorescence microscope.
3
Immunofluorescence Staining of αSMA
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Cells were fixed in PBS containing 4% formaldehyde, permeabilized in PBS containing 0.1% Triton X-100, immunostained with a rabbit anti-αSMA primary antibody (Abcam, Cambridge, UK), and labeled with a secondary antibody conjugated with an Alexa Fluor dye (Life Technologies). Nuclei were stained with TO-PRO-3 iodide (Life Technologies). Fluorescence was detected using a Carl Zeiss LSM700 laser scanning confocal microscope (Prenzlauer, Berlin, Germany).
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