Pdgfrα h2bgfp

Manufactured by Jackson ImmunoResearch

Pdgfrα-H2BGFP is a fluorescent protein fusion that labels nuclei expressing the platelet-derived growth factor receptor alpha (Pdgfrα) gene. It allows for the visualization and identification of Pdgfrα-expressing cells.

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Lab products found in correlation

Ng2 creert2, by Jackson ImmunoResearch (1 mentions) Plp1 creert, by Jackson ImmunoResearch (1 mentions) Axin2lacz, by Jackson ImmunoResearch (1 mentions) Sox2egfp, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

PdgfRα (2 citations)

2 protocols using pdgfrα h2bgfp

Mice Genetic Strains for Neurodevelopment

Cited 2 times

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All animal experiments were approved by the University of Tasmania Animal Ethics (A0013741, A0016151 and A0018606) and Institutional Biosafety Committees and were carried out in accordance with the Australian code of practice for the care and use of animals for scientific purposes. Pdgfrα-H2BGFP (stock # 007669) [Pdgfrα-histGFP^{93 (link),94 (link)}], Sun1-eGFP (stock # 021039)^{95 (link)}, Ng2-CreER^T2 (stock # 008538)^{8 (link)} and PLP1-CreERT (stock # 005975)^{96 (link)} mouse lines were purchased from Jackson Laboratories. Postnatal day (P) 0-2 Pdgfrα-histGFP neonates were genotyped using a BlueStar flashlight (Nightsea, Lexington USA) to detect GFP expression in the brain, paws and ears. Mice expressing Cre-recombinase or eGFP were genotyped by PCR^{97 (link)} using the following primer sequences: Cre 5′ CAGGT CTCAG GAGCT ATGTC CAATT TACTG ACCGTA and Cre 3′ GGTGT TATAAG CAATCC CCAGAA; GFP 5′ CCCTG AAGTTC ATCTG CACCAC and GFP 3′ TTCTC GTTGG GGTCT TTGCTC. Mice were maintained on a C57BL/6 background and inter-crossed to generate male and female offspring for experimental use. Mice were weaned >P30 to ensure appropriate myelin development, were group housed with same-sex littermates in Optimice micro-isolator cages (Animal Care Systems, Colorado, USA), and were maintained on a 12 h light/dark cycle at 20 °C with uninhibited access to food and water.

Zhen Y., Cullen C.L., Ricci R., Summers B.S., Rehman S., Ahmed Z.M., Foster A.Y., Emery B., Gasperini R, & Young K.M. (2022). Protocadherin 15 suppresses oligodendrocyte progenitor cell proliferation and promotes motility through distinct signalling pathways. Communications Biology, 5, 511.

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Genetic Lineage Tracing in Mice

Cited 1 time

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PDGFRαCreER mice (Collins et al., 2011) were bred to β-cat^fl/fl (Brault et al., 2001) and β-cat^fl(Ex3/+)) (Harada et al., 1999 (link)) mice to generate PDGFRαCreER;β-cat^fl/fl and PDGFRαCreER;β-cat^fl(Ex3/+) mice respectively. Axin2CreER (van Amerongen et al., 2012 (link)) mice were bred to Gt(ROSA)26Sortm4(ACTB-tdTomato–eGFP)Luo/J (mTmG) (Muzumdar et al., 2007 (link)). Reporter lines used to visualize Cre-recombination Gt(ROSA)26Sortm4(ACTB-tdTomato–eGFP)Luo/J (mTmG), Axin2LacZ (Lustig et al., 2002), PDGFRαH2BGFP (Hamilton et al., 2003 (link)) Sox2eGFP (Arnold et al., 2011 (link)) mice were obtained from Jackson Laboratory. R26Fucci2aR reporter mice were used to visualize the cell cycle phases (Mort et al., 2014 (link)). A random population of both male and female mice were used for all experiments, as it was not possible to sex the animals due to developmental age. All procedures involving animal subjects were performed under the approval of the Institutional Animal Care and Use Committee of the Yale School of Medicine.

Gupta K., Levinsohn J., Linderman G., Chen D., Sun T.Y., Dong D., Taketo M.M., Bosenberg M., Kluger Y., Choate K, & Myung P. (2018). Single-cell analysis reveals a hair follicle dermal niche molecular differentiation trajectory that begins prior to morphogenesis. Developmental cell, 48(1), 17-31.e6.

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