MDM and astrocyte cells were seeded at 5 × 104 cells per well in a 96-well plate. Each sample was plated in triplicate, and cell-free media was used for background measurements. The cells were treated with their respective treatments and incubated for 5 d. CellTiter-Glo reagent Luminescence Viability kit (Promega; Madison, WI, USA) and the GLOMAX Multidetection System (Promega) were used to measure the luminescence and assess cell viability. Two plate readings were recorded, and the averages of both readings were calculated.
Celltiter glo reagent luminescence viability kit
Manufactured by Promega
Sourced in United States
The CellTiter-Glo reagent Luminescence Viability kit is a cell viability assay that quantifies the amount of adenosine triphosphate (ATP) present in a sample. It is used to determine the number of metabolically active cells in culture.
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Lab products found in correlation
3 protocols using celltiter glo reagent luminescence viability kit
1
Luminescence-Based Cell Viability Assay
2
Cell Viability Assay with U1 Cells
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Approximately 5 × 104 U1 cells in fresh RPMI media were seeded into a 96 well plate, followed by treatment. Cells were incubated for 5 days and assessed for cell viability using CellTiter-Glo reagent Luminescence Viability kit (Promega) according to the manufacturer’s instructions. Luminescence was measured using the GLOMAX Multidetection System (Promega). Assays were conducted using U1 cell lines in biological triplicate with fresh RPMI media as background and used to normalize values.
3
Cytotoxicity Screening of Inhibitors
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Fifty thousand cells in fresh media were plated in triplicate on a 96-well plate, followed by treatment with inhibitors. Cells were incubated for 48 h and inhibitor treatments were assessed for cytotoxicity using Cell-Titer Glo reagent Luminescence Viability Kit (Promega; Madison, WI, USA) according to the manufacturer’s instructions. RPMI medium alone was used as background in order to normalize values.
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