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Micro volume plate

Manufactured by Agilent Technologies
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The Micro-Volume plate is a laboratory instrument designed for the precise measurement of small sample volumes. It enables accurate and efficient analysis of limited sample quantities. The core function of the Micro-Volume plate is to facilitate the quantification and characterization of analytes in a wide range of applications.

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Lab products found in correlation

Epoch plate reader, by Agilent Technologies (2 mentions) Purelink rna mini kit, by Thermo Fisher Scientific (2 mentions) Hiseq 2000 system, by Illumina (1 mentions) Rna 6000 pico kit, by Agilent Technologies (1 mentions) Rneasy column, by Qiagen (1 mentions) Synergy 2, by Agilent Technologies (1 mentions) Truseq stranded mrna library prep kit, by Illumina (1 mentions) Bioanalyzer 2100 system, by Agilent Technologies (1 mentions) Tc20 automated cell counter, by Bio-Rad (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) Fetal bovine serum (fbs), by STEMCELL (1 mentions) Bio gen pro200 homogenizer, by PRO Scientific (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Improm 2 reverse transcription system, by Promega (1 mentions)

Spelling variants of this product

Take3 Micro-Volume Plate (95 citations) Take3 micro-volume plate accessory (5 citations) Take3 Micro-Volume Plate Reader (3 citations) Take3 Micro-Volume plate adapter (2 citations)

5 protocols using micro volume plate

1

Robust RNA-seq Library Preparation

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Biological replicates for each cell line were obtained by independently growing cells in duplicate. Total RNA was purified from ~1 × 107 logarithmically growing cells using Qiagen (Valencia, CA) RNeasy columns per manufacturer’s instructions including on-column DNAse digestion. RNAs were quantified using a Take3 Micro-Volume plate in a Biotek (Winooski, VT) Synergy2 plate reader and their integrity confirmed using the Agilent RNA 6000 Pico Kit and the Agilent (Santa Clara, CA) 2100 Bioanalyzer System. 500 ng of total RNA with an RNA Integrity Number (RIN) greater than 7 were used to prepare sequencing libraries with the Illumina (San Diego, CA) TruSeq Stranded mRNA Library Prep Kit. Libraries were sequenced with an Illumina HiSeq 2000 System at the UCCC Genomics Core.
Sullivan K.D., Lewis H.C., Hill A.A., Pandey A., Jackson L.P., Cabral J.M., Smith K.P., Liggett L.A., Gomez E.B., Galbraith M.D., DeGregori J, & Espinosa J.M. (2016). Trisomy 21 consistently activates the interferon response. eLife, 5, e16220.
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2

Bone Marrow RNA Extraction Protocol

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Bone marrow was flushed from the right femur using a 19 g needle and 1 mL Iscove’s Modified Dulbecco’s Medium (IMDM) with 2% FBS (STEMCELL Technologies, Inc., Cambridge, MA). Bone marrow cellularity and viability was assessed using 0.4% Trypan blue staining on a TC20 automated cell counter (Bio-Rad, Hercules, CA).
The bone marrow was then centrifuged at 5,000 rpm in a microcentrifuge (Beckman-Coulter, Brea, CA) for 5 minutes. The supernatant was aspirated and preserved. Then 650 μl of RNA lysis buffer with 2-mercaptoethanol (Sigma), at a dilution of 10 μl 2-mercaptoethanol per mL lysis buffer, was added to the cell pellet and homogenized using a Bio-Gen Pro200 Homogenizer (PRO Scientific, Oxford CT). RNA was extracted from bone marrow and 30 mg liver using Purelink RNA mini kit (Invitrogen, Waltham, MA) following the manufacturer’s instructions. RNA concentrations and purity were determined by measuring the 260/280 ratio in a BioTek micro-volume plate on an EPOCH plate reader (BioTek Instruments, Winooski, VT).
Kelly L.S., Munley J.A., Pons E.E., Kannan K.B., Apple C.G., Thompson C.W., Efron P.A, & Mohr A.M. (2022). Mechanisms of improved erythroid progenitor growth with removal of chronic stress after trauma. Surgery, 172(2), 759-765.
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3

Liver RNA Extraction and Quantification

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RNA was extracted from 30 mg liver using Purelink RNA mini kit (Invitrogen, Waltham, MA) following the manufacturer’s instructions. RNA concentrations and purity were determined by measuring the 260/280 ratio in a BioTek micro-volume plate on an EPOCH plate reader (BioTek Instruments, Winooski, VT).
Kelly L.S., Munley J.A., Pons E.E., Kannan K.B., Apple C.G., Thompson C.W., Efron P.A, & Mohr A.M. (2022). Mechanisms of improved erythroid progenitor growth with removal of chronic stress after trauma. Surgery, 172(2), 759-765.
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4

Determination of USP17 Protein Concentration

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The concentration of pure USP17 or USP17-Cys89Ser was determined using a Take3 Micro-Volume plate with a Biotek Synergy H1 plate reader. The extinction coefficient of purified USP17 was experimentally determined to be 58,104 M-1cm-1 for wild-type, and 58,338 M-1cm-1 for USP17-Cys89Ser as described by von Hippel et al [16 (link)].
Hjortland N.M, & Mesecar A.D. (2016). Steady-state kinetic studies reveal that the anti-cancer target Ubiquitin-Specific Protease 17 (USP17) is a highly efficient deubiquitinating enzyme. Archives of biochemistry and biophysics, 612, 35-45.
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5

MCU Silencing Gene Expression Analysis

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For the gene expression analysis of MCU silencing, after 96 h of transfection, total RNA was isolated from H9c2 cells by homogenization in TriReagent™ following manufacturer's instructions. The RNA quantification and purity assessment was performed with a Take3™ Micro-Volume plate used in the microplate spectrophotometer Synergy™ HT (BioTek® Instruments, Winooski, VT, USA). From one microgram of total RNA of each sample, cDNA was synthesized with the ImProm-II™ Reverse Transcription System (Promega®, Madison, WI, USA) and 5 ng was analyzed by qRT-PCR SensiFast™ SYBR® Lo-Rox Kit (Bioline®, London, UK). The housekeeping gene β-actin was used to normalize all data. Real-time PCR primer sequences to amplify a fragment of 158 bp of CCDC109A (MCU) gene are the following: fw, 5′-CACACAGTTTGGCATTTTGG-3′, and rv, 5′-TGTCTCTGGCTTCAGGATAA-3′. The primer sequences to amplify a fragment of 110 bp of β-actin are fw, 5′-GAAAAGATGACCCAGATCATG-3′, and rv, 5′-ATCACAATGCCAGTGGTAC-3′. Comparing expression analysis in siRNA-MCU cells to siRNA-Neg cells was performed using 2−ΔΔCt method.
Oropeza-Almazán Y., Vázquez-Garza E., Chapoy-Villanueva H., Torre-Amione G, & García-Rivas G. (2017). Small Interfering RNA Targeting Mitochondrial Calcium Uniporter Improves Cardiomyocyte Cell Viability in Hypoxia/Reoxygenation Injury by Reducing Calcium Overload. Oxidative Medicine and Cellular Longevity, 2017, 5750897.
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