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Dmla microscope

Manufactured by Leica
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Sourced in Germany

The Leica DMLA is a high-performance microscope designed for advanced laboratory applications. It features a sturdy, ergonomic design and offers optical excellence with a wide range of magnification capabilities. The DMLA is a versatile instrument suitable for various scientific and research purposes.

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Lab products found in correlation

Immpress system, by Vector Laboratories (3 mentions) P akt, by Abcam (2 mentions) P erk and p smad2, by Cell Signaling Technology (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Nanozoomer 2.0 rs, by Hamamatsu Photonics (1 mentions) Ndp view2, by Hamamatsu Photonics (1 mentions) Ccd camera, by Leica (1 mentions) Ab58810, by Abcam (1 mentions) Xylene, by Avantor (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Biocoat growth factor reduced matrigel invasion chamber, by Corning (1 mentions) Rm2165 microtome, by Leica (1 mentions) Lr white, by Electron Microscopy Sciences (1 mentions) Rm2255 microtome, by Leica (1 mentions) Toluidine blue o, by Merck Group (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Krystalon, by Merck Group (1 mentions) Axiocam hrc digital camera, by Zeiss (1 mentions) Axiocam hrc camera, by Zeiss (1 mentions)

Close spelling variants of this product

DMLA full automatic microscope Leica DMLA light microscope

15 protocols using dmla microscope

1

Quantitative Histological Analysis of Samples

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Where indicated, slides were scanned by the digital slide scanner NanoZoomer 2.0-RS (Hamamatsu) allowing an overall view of the samples. Images were digitally captured from the scanned slides using the NDP.view2 software (Hamamatsu). All quantifications from the histological analyses were performed by counting 10 different fields on the scanned slides. Other slides were analyzed using a Leica DMLA microscope, in particular to visualize images at 100X magnification, and images were captured with a Leica DFC450 C digital microscope camera. Immunochemical stainings were interpreted simultaneously and independently by at least two investigators (FM, LC, or FG).
Meuris F., Carthagena L., Jaracz-Ros A., Gaudin F., Cutolo P., Deback C., Xue Y., Thierry F., Doorbar J, & Bachelerie F. (2016). The CXCL12/CXCR4 Signaling Pathway: A New Susceptibility Factor in Human Papillomavirus Pathogenesis. PLoS Pathogens, 12(12), e1006039.
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2

Quantifying White Adipose Tissue in Mice

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Five-day-old WT and MTKO mice were sacrificed by blood removal and cervical dislocation under isoflurane anesthesia, and their dorsal-ventral subcutaneous fat pads were excised and weighed. All efforts were made to minimize suffering. The percent WAT weight was calculated by dividing the fat mass by the body weight. WAT samples collected from the mice were fixed in Mildform 15NM (Wako Pure Chemicals, Tokyo, Japan) and embedded in paraffin. WAT sections (3 μm thick) were prepared on silane-coated slides and stained with hematoxylin and eosin (HE). Digital images of the WAT sections were obtained using a Leica DMLA microscope with a CCD camera (Leica, Wetzlar, Germany).
Kadota Y., Toriuchi Y., Aki Y., Mizuno Y., Kawakami T., Nakaya T., Sato M, & Suzuki S. (2017). Metallothioneins regulate the adipogenic differentiation of 3T3-L1 cells via the insulin signaling pathway. PLoS ONE, 12(4), e0176070.
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3

Immunohistochemistry of PDL1 and PDL2

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10% formalin-fixed tissues were mounted and embedded in paraffin and sectioned (5µm) onto slides. These were then rehydrated in xylene (VWR), followed by increasing dilutions of ethanol (100%, 75%, 50% and 25%). Antigen retrieval was performed by boiling for 25mins in 10mM Sodium Citrate Buffer at a pH of 6.0. Sections were then transferred to PBS (Gibco), excess liquid removed and staining performed using rabbit anti-mouse PDL1 (AbCam ab58810) or anti-mouse PDL2 (AbCam ab21239). Secondary staining with donkey alexa-488 conjugated anti-rabbit (Invitrogen) was then performed. Sections were mounted in anti-fade mounting media containing DAPI (Invitrogen) and imaged using a Leica DMLA microscope.
Murphy S.F., Schaeffer A.J., Done J.D., Quick M.L., Acar U, & Thumbikat P. (2017). Commensal bacterial modulation of the host immune response to ameliorate pain in a murine model of chronic prostatitis. Pain, 158(8), 1517-1527.
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4

Invasion and Migration Assay with Matrigel

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A 24-well BioCoat growth factor reduced Matrigel invasion chamber (Corning, #354483) was used for invasion and migration assays. Specifically, 5 × 104 UM-SCC-1 or 1 × 105 MDA1586 stable cells were seeded in the upper chambers and incubated with serum-free DMEM; DMEM supplemented with 10% fetal bovine serum was added to the lower chamber. The plates were placed in an incubator (37 °C) under 5% CO2 for 24 h. The cells in the upper chamber were removed using cotton swabs, and the cells that had invaded and migrated to the lower chamber were stained with a solution of 0.2% crystal violet in 25% methanol. Then the chamber membranes were mounted on glass slides and examined with a Leica DMLA microscope. At least three random 100× fields were selected for each cell line. Cell numbers were counted and analyzed using Image J software (NIH).
Zhao M., Wang T., Gleber-Netto F.O., Chen Z., McGrail D.J., Gomez J.A., Ju W., Gadhikar M.A., Ma W., Shen L., Wang Q., Tang X., Pathak S., Raso M.G., Burks J.K., Lin S.Y., Wang J., Multani A.S., Pickering C.R., Chen J., Myers J.N, & Zhou G. (2024). Mutant p53 gains oncogenic functions through a chromosomal instability-induced cytosolic DNA response. Nature Communications, 15, 180.
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5

Immunohistochemical Analysis of Tumor Samples

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Tumors were fixed in 10% neutral buffered formalin. Samples were then transferred to 75% ethanol and embedded in paraffin. Histologic sections were stained with hematoxylin and eosin or processed for immunohistochemical analysis. Tissue sections used for immunohistochemical analysis were deparaffinized and then rehydrated following xylene and alcohol series. Antigen retrieval was performed in 100 mM sodium citrate (pH 6.0), and endogenous peroxidase was blocked with 1% hydrogen peroxide. Then, tissue sections were incubated with primary antibodies for p-Akt (Epitomics, Burlingame, CA); p-Erk and p-Smad2 (Cell Signaling Technology, Danvers, MA); CD45, Gr-1, F4/80 and Nk1 (BioLegend, San Diego, CA); CD3, α–SMA and Ki67 (Thermo Scientific, Rockford, IL); p16 (Santa Cruz Biotechnology, Santa Cruz, CA). Primary antibodies were detected using the ImmPRESS system (Vector Laboratories, Burlingame, CA). Slides were visualized on a Leica DMLA microscope (Leica Microsystems, Buffalo Grove, IL), and images were captured with a Hamamatsu ORCA-ER camera (Shizuoka, Japan).
Savar A., Acin S., Gonzalez C.L., El-Sawy T., Mejia O., Li Z., Esmaeli B., Lacy-Hulbert A., El-Naggar A., McCarty J.H, & Caulin C. (2014). Loss of Epithelial p53 and αv Integrin Cooperate through Akt to Induce Squamous Cell Carcinoma yet Prevent Remodeling of the Tumor Microenvironment. Oncogene, 34(4), 516-524.
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6

Histological Analysis of AZ-C Tissue

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Tissue containing the AZ-C after 0, 24, and 48 h of ACC treatment was manually dissected using a razor blade in 0.5 cm3 portions. These samples were fixed overnight at 4°C in a 4% (w/v) paraformaldehyde-PBS solution. After fixation, samples were washed with PBS, dehydrated in a graded ethanol series and embedded in LR White (Electron Microscopy Sciences). Longitudinal sections of the calyx button area (1 μm thickness) were cut with a Leica RM2165 microtome and placed on glass slides. Slides were further stained with PAS (Sigma-Aldrich) and mounted with DPX Mountant (Fluka). Observations were performed on a Leica DMLA microscope (Leica Microsystems) and images were processed with Leica ASMLD Version 4.0 software.
Merelo P., Agustí J., Arbona V., Costa M.L., Estornell L.H., Gómez-Cadenas A., Coimbra S., Gómez M.D., Pérez-Amador M.A., Domingo C., Talón M, & Tadeo F.R. (2017). Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus. Frontiers in Plant Science, 8, 126.
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7

Senescence Morphology in N. benthamiana

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Flowers from N. benthamiana plants inoculated with the empty clbv3’ vector and clbv3’-NbenIDA construct were sampled at developmental stages 5 (onset of corolla senescence with margins of the corolla limb lobes curling inwards) and 6 (corolla limb completely contracted and brown and corolla tube drying).
Samples containing the capsule and the base of the corolla tube attached to the flower receptacle were fixed and embedded in LR White resin (London Resin Co., Woking, Surrey, UK) according to [56 (link)]. Longitudinal sections (about 1 μm thick) were cut with a Leica RM2255 microtome (Leica Microsystems, Wetzlar, Germany) using glass knives and fixed to microscope slides. Sections were stained with Toluidine Blue O (CI 52040; Merck, Darmstadt, Germany) after [57 (link)] and examined and photographed with a Leica DM LA microscope (Leica Microsystems, Wetzlar, Germany).
Ventimilla D., Velázquez K., Ruiz-Ruiz S., Terol J., Pérez-Amador M.A., Vives M.C., Guerri J., Talon M, & Tadeo F.R. (2021). IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers. BMC Plant Biology, 21, 226.
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8

Histological Analysis of Cardiac Tissue

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Paraffin-embedded hearts were sliced into 5-μm thick sections and used for subsequent histological analyses. Hematoxylin and eosin (H&E) and picrosirius red staining of paraffin-embedded heart sections were visualized using a Leica DMLA microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Retiga 1300i FAST 1394 CCD camera (OImaging, Surrey, BC, Canada) as described previously [2 (link)]. Representative images from each sample are shown. For wheat-germ-agglutinin (WGA) staining, sections were treated with WGA conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, Mississauga, ON, Canada); then, nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) using ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific). The cross-sectional area of cardiomyocytes was quantified using imagej software (National Institutes of Health, Bethesda, MD, USA) as described previously [16 (link)].
Maayah Z.H., Alam A.S., Takahara S., Soni S., Ferdaoussi M., Matsumura N., Zordoky B.N., Eisenstat D.D, & Dyck J.R. (2021). Resveratrol reduces cardiac NLRP3-inflammasome activation and systemic inflammation to lessen doxorubicin-induced cardiotoxicity in juvenile mice. FEBS letters, 595(12), 1681-1695.
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9

Toluidine Blue Mast Cell Staining

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Toluidine blue staining, a metachromatic dye, stain mast cells red-purple (metachromatic staining) and the background blue (orthochromatic staining) for the identification of mast cells. Briefly, 5 μm-thick sections were stained using fresh 0.1% toluidine blue for 2–3 mins, washed thrice using distilled water, dehydrated in 50, 70, 95, and 100% of ethanol, cleared in xylene, and mounted with Krystalon (EMD Millipore, MA, USA). Bright-field images were taken on a Leica DMLA microscope (Leica Microsystems, Buffalo Grove, IL) using a QImaging MicroPublisher 3.3 RTV camera (Teledyne Photometrics, Tucson, AZ) and analyzed on Micromanager, an open-source microscopy software (35 (link)).
Pattabiraman G., Liu Z., Paul M., Schaeffer A.J, & Thumbikat P. (2022). mMCP7, a Mouse Ortholog of δ Tryptase, Mediates Pelvic Tactile Allodynia in a Model of Chronic Pelvic Pain. Frontiers in Pain Research, 2, 805136.
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10

Cardiac Fibrosis Microscopy Workflow

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Masson’s trichrome staining and picrosirius red staining of paraffin-embedded left ventricular human heart sections taken mid-papillary were visualized using a Leica DMLA microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Retiga 1300i FAST 1394 CCD camera (OImaging, Surrey, BC, Canada), as described previously [19 (link)]. Three representative images were taken from each sample and densitometric analysis was performed using the ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Uddin G.M., Zhang L., Shah S., Fukushima A., Wagg C.S., Gopal K., Al Batran R., Pherwani S., Ho K.L., Boisvenue J., Karwi Q.G., Altamimi T., Wishart D.S., Dyck J.R., Ussher J.R., Oudit G.Y, & Lopaschuk G.D. (2019). Impaired branched chain amino acid oxidation contributes to cardiac insulin resistance in heart failure. Cardiovascular Diabetology, 18, 86.
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