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C3 cell stand

Manufactured by Bioanalytical Systems
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Sourced in United States

The C3 cell stand is a laboratory equipment designed to hold and position cell culture vessels during various experimental procedures. It provides a stable and adjustable platform to support cell culture dishes, plates, or flasks, facilitating convenient access and manipulation of the samples.

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Lab products found in correlation

Mw 1032, by Bioanalytical Systems (2 mentions) Mf 2056, by Bioanalytical Systems (2 mentions) Multipalmsens4, by PalmSens (2 mentions) Lutein, by Thermo Fisher Scientific (1 mentions) Methylene blue, by Thermo Fisher Scientific (1 mentions) β cyclodextrin, by Merck Group (1 mentions)

4 protocols using c3 cell stand

1

Electrochemical Characterization of Bioactive Compounds

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Beta-carotene (97%), lutein (90%), and methylene blue were purchased from Acros organics. β-cyclodextrin was purchased from Sigma Aldrich. All other reagents were analytical grade and purchased from Fisher Scientific. Phosphate buffer saline solution (PBS) was produced using a standard protocol and made in-house to a pH of 7.3. 0.1 M tetrabutylammonium hexafluorophosphate (NBu4PF6) electrolyte was added for measurements in dimethyl sulfoxide (DMSO).
All electrochemical tests were run on a Gamry Reference 600 potentiostat using a a BASi C3 cell stand faraday cage. The electrode setup included a glassy carbon or platinum working electrode and a platinum auxiliary electrode. A Ag/AgNO3 reference electrode was used for DMSO electrolytes, and a Ag/AgCl reference electrode was used for aqueous electrolytes. Nitrogen was bubbled through the solution to create an anoxic environment. Stirring occurred in between electrochemical data points, but not during the
Thompson G., Marnoto S, & Halpern J.M. (2017). Proper Controls to Electrochemically Evaluate Carotenoids using β-Cyclodextrin Modified Surfaces. ECS transactions, 80(10), 1177-1187.
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2

Electrochemical Detection of SARS-CoV-2 in Saliva

Cited 1 time
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Electrochemical tests were carried out with a three-electrode cell stand (BASi®, C-3 Cell Stand, West Lafayette, IN, United States) connected to a benchtop potentiostat/impedance analyzer (PalmSens®, MultiPalmSens4, Houten, Netherlands). A platinum wire (7.5 cm) was used as auxiliary electrode (BASi®, MW1032, West Lafayette, IN, United States), Ag/AgCl (3M KCl) was used as reference electrode (BASi®, MF 2056, West Lafayette, IN, United States), and an ACE2-functionalized electrode (LIG-nPt-hACE2) was used as working electrode.
Electrochemical impedance spectroscopy (EIS) was performed in a non-Faradaic mode by immersing the electrodes in a 2X (w/v) solution of sodium chloride (38.3 g/L)/sodium bicarbonate (11.6 g/L) buffer supplemented with 10% (v/v) pooled saliva (pH 8) at 22°C and 1 atm. EIS spectra were obtained within the frequency range of 0.01 Hz–10,000 Hz, using an AC amplitude of 0.08 V and a DC voltage of 0.36 V.
Titration experiments were conducted to evaluate the EIS response of the LIG-nPt-hACE2 biosensors to clinically relevant concentration levels of SARS-CoV-2 in saliva (Bar-On et al., 2020 (link)). The analyte solution (attenuated virus suspended in pooled saliva) was drop-casted onto the working electrode and incubated for 10 min at room temperature. After incubation, the electrodes were rinsed and then tested using EIS.
Moreira G., Casso-Hartmann L., Datta S.P., Dean D., McLamore E, & Vanegas D. (2022). Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva. Frontiers in sensors, 3, 917380.
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3

Electrochemical Detection of SARS-CoV-2 in Saliva

Cited 1 time
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Electrochemical tests were carried out with a three-electrode cell stand (BASi®, C-3 Cell Stand, West Lafayette, IN, United States) connected to a benchtop potentiostat/impedance analyzer (PalmSens®, MultiPalmSens4, Houten, Netherlands). A platinum wire (7.5 cm) was used as auxiliary electrode (BASi®, MW1032, West Lafayette, IN, United States), Ag/AgCl (3M KCl) was used as reference electrode (BASi®, MF 2056, West Lafayette, IN, United States), and an ACE2-functionalized electrode (LIG-nPt-hACE2) was used as working electrode.
Electrochemical impedance spectroscopy (EIS) was performed in a non-Faradaic mode by immersing the electrodes in a 2X (w/v) solution of sodium chloride (38.3 g/L)/sodium bicarbonate (11.6 g/L) buffer supplemented with 10% (v/v) pooled saliva (pH 8) at 22°C and 1 atm. EIS spectra were obtained within the frequency range of 0.01 Hz–10,000 Hz, using an AC amplitude of 0.08 V and a DC voltage of 0.36 V.
Titration experiments were conducted to evaluate the EIS response of the LIG-nPt-hACE2 biosensors to clinically relevant concentration levels of SARS-CoV-2 in saliva (Bar-On et al., 2020 (link)). The analyte solution (attenuated virus suspended in pooled saliva) was drop-casted onto the working electrode and incubated for 10 min at room temperature. After incubation, the electrodes were rinsed and then tested using EIS.
Moreira G., Casso-Hartmann L., Datta S.P., Dean D., McLamore E, & Vanegas D. (2022). Development of a Biosensor Based on Angiotensin-Converting Enzyme II for Severe Acute Respiratory Syndrome Coronavirus 2 Detection in Human Saliva. Frontiers in sensors, 3, 917380.
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4

Cyclic Voltammetry of Modified Glassy Carbon

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Cyclic voltammetry was carried out using a BASi Epsilon potentiostat with a C3 cell stand. A three-electrode system was employed comprising a modified glassy carbon electrode as the working electrode, Pt wire counter, and Ag/AgCl-saturated NaCl reference electrode. The standard buffer solution was a 0.1 M sodium phosphate buffer at pH 7.0. All potentials are quoted versus the normal hydrogen electrode (NHE), calculated as Ag/AgCl +196 mV. The general scan procedure was as follows; scan rate: 10 mV s−1, initial potential: −600 mV s−1, switching point 1: 400 mV, switching point 2: −1500 mV, quiet time: 10 s, total number of scans: 5. The final cycle is reported.
Rapson T.D., Ju H., Marshall P., Devilla R., Jackson C.J., Giddey S, & Sutherland T.D. (2020). Engineering a solid-state metalloprotein hydrogen evolution catalyst. Scientific Reports, 10, 3774.
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