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Ti eclipse inverted optical microscope

Manufactured by Nikon
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Sourced in Japan

The Ti-Eclipse is an inverted optical microscope designed for laboratory use. It features a stable and precise optical system that enables high-resolution imaging of samples. The microscope is equipped with various illumination options and supports a range of objectives to accommodate diverse imaging requirements.

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Lab products found in correlation

Nis elements software, by Nikon (2 mentions) A1 confocal system, by Nikon (2 mentions) Ethd 1, by Thermo Fisher Scientific (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Air therm heater, by World Precision Instruments (1 mentions) Ti sh w, by Nikon (1 mentions) Cary 620, by Agilent Technologies (1 mentions) F 7000 fluorescence spectrophotometer, by Hitachi (1 mentions) Nanowizard 2 afm, by Nikon (1 mentions) Iraffinity 1, by Shimadzu (1 mentions) Cm200, by Philips (1 mentions)

6 protocols using ti eclipse inverted optical microscope

1

Bacteria Fixation and Microscopy

Cited 1 time
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Bacteria were fixed with paraformaldehyde, as previously described7 (link), then mounted on an agarose pad before imaging with a Nikon Ti Eclipse inverted optical microscope in phase contrast mode.
Turner R.D., Mesnage S., Hobbs J.K, & Foster S.J. (2018). Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology. Nature Communications, 9, 1263.
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2

Assessing Neuronal Responses to Compression

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For all compression experiments, neuron viability and geometry were assessed using calcein AM (Life Technologies, Grand Island, NY), and acute alterations in plasma membrane permeability following compressive loading were assessed by evaluating uptake of Alexa Fluor 568 Hydrazide (AFH) (Life Technologies). Three-dimensional image volumes of neurons immediately after mechanical insult were acquired using a temperature-controlled Nikon A-1 confocal system mounted on a Ti-Eclipse inverted optical microscope controlled by NIS–Elements Nikon software (Nikon, Tokyo, Japan). Control experiments were conducted to confirm neuron population viability in the collagen hydrogels through 9 DIV (Supplementary Fig. S1) using calcein AM as a live cell stain and Ethidium homodimer–1 (EthD-1; Life Technologies) as a dead cell stain. Control experiments to induce cell membrane poration were performed by introducing 0.1% Triton X-100 after staining with calcein AM and AFH.
Bar-Kochba E., Scimone M.T., Estrada J.B, & Franck C. (2016). Strain and rate-dependent neuronal injury in a 3D in vitro compression model of traumatic brain injury. Scientific Reports, 6, 30550.
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3

Confocal Imaging of Fluorescent Spheres

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Three-dimensional image stacks were acquired using a Nikon A-1 confocal system mounted on a TI Eclipse inverted optical microscope controlled by NI-Elements Nikon Software. A 40× plan fluor air objective mounted on a piezo objective positioner was used for all the experiments, which allowed imaging at speeds up to 30 frames per second. Green 0.5 μm fluorescent microspheres were embedded into the substrate and excited with an Argon (488 nm) laser. 512 × 512 × Z voxels (102μm × 102 μm × Z) confocal volume stacks were recorded every 100 seconds with Z ~ 128 voxels (38 μm), illustrated in Fig. 1(b). To ensure physiological imaging conditions within the imaging chamber, temperature and pH were strictly maintained at 37 °C and ~ 7.4 during time lapse recording as previously described6 , 7 . The outline of the cell was estimated from phase contrast microscopy images.
Toyjanova J., Hannen E., Bar-Kochba E., Darling E.M., Henann D.L, & Franck C. (2014). 3D Viscoelastic Traction Force Microscopy. Soft matter, 10(40), 8095-8106.
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4

Live-Cell Laser Cavitation Imaging

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For all live-cell experiments and controls, three-dimensional image stacks of cells immediately before and immediately following laser-induced cavitation were acquired using a Nikon A-1 laser scanning confocal or multiphoton system, mounted on a Ti-Eclipse inverted optical microscope controlled by NIS-elements software (Nikon, Tokyo, Japan). To maintain physiological conditions, a custom-made environmental chamber was built around the microscope and thermally-controlled at 37 C with a closed-loop Air-Therm heater (World Precision Instruments, Sarasota, FL). Samples in 48-well glass-bottomed plates were secured using a well plate holder (Ti-SH-W; Nikon), which was allowed to thermally equilibrate before imaging to avoid thermal drift in imaging locations. All cavitation events were performed 600 µm above the top of the glass coverslip, or approximately at the mid-plane of the gels.
Estrada J.B., Cramer H.C., Scimone M.T., Buyukozturk S., & Franck C. (2021). Neural cell injury pathology due to high-rate mechanical loading.
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5

FTIR and Dark-field Imaging of Breast and Ovarian Tissue

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Both FTIR and dark-field microscopy are used to image unstained tissue cores from breast and ovarian TMAs. Tissue sections were prepared using standard protocols11 (link) for FTIR imaging. 5 μm thick tissue sections from FFPE blocksmounted on IR transparent windows of CaF2 were deparaffinized for imaging, first imaged with FTIR imaging system (Agilent 670 spectrometer coupled to a Cary 620 microscopy system) and then with a dark-field microscope (Nikon Eclipse Ti inverted optical microscope). Agilent Cary 620 FTIR has 15 × 0.62NA and 128 × 128 pixels focal plane array (FPA) detector. We collected mid-IR HS images of tissue sections using standarddefinition (SD) mode with 5.5 μm pixel size and 8 cm−1 spectral resolution in the spectral range of 1000 to 3900 cm−1.
Tissue sections were imaged with a Nikon inverted optical microscope with a 10×, 0.4NA objective in the dark-field mode. A dark-field condenser transmits a hollow cone of light and blocks light from within a disk around the optical axis. In the presence of a sample, scattered light is collected by the objective forming a bright image against a dark background. Based on the Rayleigh criteria, the diffraction-limited spatial resolution of dark-field images collected in the visible range (400 to 700 nm) is significantly higher than FTIR images in the fingerprint region (2.5 to 12 μm).
Mankar R., Gajjela C.C., Shahraki F.F., Prasad S., Mayerich D, & Reddy R. (2021). Multi-modal image sharpening in fourier transform infrared (FTIR) microscopy. The Analyst, 146(15), 4822-4834.
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6

MOF-based Fluorescent Sensor for DNT

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All solvents and starting materials for synthesis were commercially available and were used as received. Fourier transform infrared spectra (FTIR) were collected using a Shimadzu IRAffinity-1 from 4000–400 cm−1. PXRD patterns were collected at room temperature using an Empyrean PANalytical diffractometer (CuKα1,2 radiation, λ1 = 1.540598 Å and λ2 = 1.544426 Å) equipped with a PIXcel 1D detector and a flat-plate sample holder in a Bragg–Brentano para-focusing optics configuration (45 kV, 40 mA). Intensity data were obtained by the step counting method (step: 0.02°) in continuous mode in the approximate range 3.0° ≤ 2θ ≤ 50°. Scanning electron microscope (SEM) images were acquired using a Philips CM-200 (200 kV). Fluorescence spectra for the characterization of the MOF and for DNT sensing were collected using a Hitachi F-7000 Fluorescence Spectrophotometer (Hitachi High Technologies, Krefeld, Germany). Fluorescence spectra of films containing [Zn2(bpdc)2(bpee)] were obtained by using a sample holder for solid samples. AFM images were acquired by means of a JPK NanoWizard II AFM functioning in contact mode, connected to a Nikon Eclipse Ti inverted optical microscope. MikroMasch HQ:XSC11/Al BS cantilevers of 0.2 N/m spring constant and 15 kHz resonant frequency were used in air conditions.
Roales J., Moscoso F.G., Gámez F., Lopes-Costa T., Sousaraei A., Casado S., Castro-Smirnov J.R., Cabanillas-Gonzalez J., Almeida J., Queirós C., Cunha-Silva L., Silva A.M, & Pedrosa J.M. (2017). Preparation of Luminescent Metal-Organic Framework Films by Soft-Imprinting for 2,4-Dinitrotoluene Sensing. Materials, 10(9), 992.
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