Polyethylene glycol mn 400

The anti-dystrophin (Mandys8), anti-sarcospan (E-2), and anti-myosin (MY-32) monoclonal antibodies were supplied by Santa Cruz Biotechnology (Dallas, Texas, USA). The anti-dystrophin antibody was conjugated with Maxpar® ¹⁵⁸Gd reagent by Fluidigm (South San Francisco, CA, USA). The anti-sarcospan and anti-myosin antibodies were conjugated in-house with Maxpar® ¹⁶²Dy and ¹⁴⁶Nd respectively according to the Fluidigm protocol. The concentration of the antibody-conjugates were determined by measuring the absorbance at 280 nm, and as per the protocol were diluted to 0.5 mg mL⁻¹ for storage at 4°C. Bloxall and mouse on mouse (M.O.M.) basic kit were purchased from Vector Laboratories (Burlingame, CA, USA), Superblock and Tween-20 from Thermo Fisher Scientific (Waltham, MA, USA) and 10x TBS from Bio-Rad (Hercules, CA, USA). 0.1% TBST was prepared from TBS and Tween-20.
Seastar Baseline nitric acid, (NO₃) and 1,000 μg mL⁻¹ standards of Dy, Gd, and Nd were supplied by Choice Analytical (Thornleigh, New South Wales, Australia). Tris-HCI (pH 7.4), ethylenediaminetetraacetic acid (EDTA; 10 mM), polyethylene glycol (Mn 400) and gelatine from bovine skin (100 mg; Type B) were purchased from Sigma Aldrich (Castle Hill NSW, Australia).
Grace Bio-Labs (Bend, OR) supplied 6 Hybriwell™ gasket (20x9.8 mm) and clear polycarbonate cover with two ports (item number 612107, depth 0.25 mm, volume 50 μL).

Gadolinium(III) nitrate hexahydrate, Tris-HCl (pH 7.4), ethylenediaminetetraacetic acid (EDTA; 10 mM), polyethylene glycol (M_n 400), and gelatin from bovine skin (100 mg; Type B) were purchased from Sigma-Aldrich (Castle Hill, NSW, Australia).
Grace Bio-Laboratories (Bend, OR, U.S.A.) supplied 6 Hybriwell gasket (20 × 9.8 mm) and clear polycarbonate cover with two ports (item number 612107, depth 0.25 mm, volume 50 μL). Ultrapure HNO₃ and a certified Gd standard were supplied by Choice Analytical (Thornleigh, New South Wales, Australia). Antidystrophin monoclonal antibody (MANDYS8, sc-58754) was supplied by Santa Cruz Biosciences (Dallas, Texas, U.S.A.). MaxPar Labeling kit was purchased from Fluidigm (South San Francisco, CA, U.S.A.). Mouse on Mouse (M.O.M.) Blocking Reagent (MKB-2213) was purchased from Vector Laboratories (Burlingame, CA, U.S.A.). Superblock (TBS; 37535) and TBS containing 0.1% Tween-20 (28360, TBS-T) were supplied by Thermofisher, (Waltham, Ma, U.S.A.).