To analyze the angiogenesis-related protein profiles in the retinas, a mouse angiogenesis array (R&D Systems, MN, USA) was performed following the manufacturer’s protocol. Briefly, the pooled mice retinas (n = 3) were homogenized in PBS using protease inhibitors and centrifuged at 10,000 × g for 5 min, and the total protein concentrations were quantified. The lysates were added to a membrane spotted with antibodies against angiogenesis-related proteins. After being incubated overnight at 4 °C, the membranes were treated with streptavidin-horseradish peroxidase and visualized using an enhanced chemiluminescence detection system (Amersham Bioscience, NJ, USA) on an image analyzer (LAS-3000, Fujifilm, Tokyo, Japan). Optical density measurements were obtained using the ImageJ software (NIH, MD, USA).
Mouse angiogenesis array
Manufactured by R&D Systems
Sourced in United States
The Mouse Angiogenesis Array is a multiplex assay that allows for the simultaneous detection and quantification of 53 angiogenesis-related proteins in mouse samples. It provides a comprehensive analysis of the angiogenic profile within the sample.
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Lab products found in correlation
5 protocols using mouse angiogenesis array
1
Angiogenesis Protein Profiling in Retinas
2
Mouse Liver Proteome Profiling
3
Mouse Angiogenesis Proteome Profiling
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To analyze the proteome profile of angiogenesis-related factors, mouse angiogenesis array (ARY015, R&D System) was incubated with fresh cellular lysates containing 1 mg protein according to the manufacturer’s instructions. The RapidStep™ ECL reagent (No.345818, Millipore) was used, and spot intensities were measured using the NIH ImageJ software (Bethesda, MD) for data capture.
4
Angiogenesis Protein Expression Profiling
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The Mouse Angiogenesis Array (R&D Systems, Inc.) was used to detect the expression of 53 angiogenesis-related proteins in the experimental groups. These Proteome Profiler Arrays were handled according to the manufacturer's protocol. Briefly, the protein extraction was done, and 100 μg of total protein was mixed with a cocktail of biotinylated detection antibodies and then incubated with a nitrocellulose membrane spotted with capture antibodies in duplicate. Protein detection antibodies bound to the capture antibody were detected using streptavidin–HRP and chemiluminescent detection reagents. The Gel Doc EZ System (Bio-Rad) was used for imaging. The mean spot pixel density was quantified using image software analysis.
5
Retinal Angiogenesis Protein Profiling
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To investigate angiogenesis-related proteins in the retinas, a mouse angiogenesis array (R&D Systems, MN, USA) was performed according to the manufacturer’s protocol. Briefly, the retinas (n = 3) were homogenized in PBS using protease inhibitors and centrifuged at 10,000 x g for 5 minutes, and 250 ug of total protein concentrations were quantified. The lysates were added to a membrane spotted with antibodies against angiogenesis-related proteins. After being incubated overnight at 4 °C, the membranes were treated with streptavidin-horseradish peroxidase and were visualized using an enhanced chemiluminescence detection system (Amersham Bioscience, NJ, USA) on an image analyzer (LAS-3000, Fujifilm, Tokyo, Japan). Optical density measurements were obtained using the ImageJ software (NIH, MD, USA).
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