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Osr60118

Manufactured by Beckman Coulter
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Sourced in United States

The OSR60118 is a laboratory instrument designed for automated blood cell analysis. It provides precise measurements of various blood cell parameters, including cell counts, cell types, and other related metrics. The core function of the OSR60118 is to perform accurate and reliable blood analysis to support clinical diagnostics and research.

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Lab products found in correlation

Osr6116, by Beckman Coulter (3 mentions) Osr6187, by Beckman Coulter (2 mentions) Au480, by Beckman Coulter (2 mentions) Beckman au480 clinical, by Beckman Coulter (1 mentions) Osr6195, by Beckman Coulter (1 mentions) Osr6234, by Beckman Coulter (1 mentions)

5 protocols using osr60118

1

Lipid Profile Measurement Protocol

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Plasma total triglycerides, total cholesterol, and high-density lipoprotein (HDL)-cholesterol concentrations were measured using commercial enzymatic kits (OSR60118, OSR6116, and OSR6187, respectively (Beckman Coulter Inc., Georgia, USA)) on a Beckman AU480 clinical analyser (Beckman Coulter Inc., Atlanta, GA, USA). Plasma low-density lipoprotein (LDL)-cholesterol concentrations were estimated using the Friedewald equation [24 (link)]:
O’Rielly R., Li H., Lim S.M., Yazbeck R., Kritas S., Ullrich S.S., Feinle-Bisset C., Heilbronn L, & Page A.J. (2020). The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice. Nutrients, 12(8), 2446.
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2

Quantification of Hepatic Triglycerides

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Hepatic triglyceride content was quantified as described previously [18 (link)] with slight modifications. Briefly, frozen liver tissues (~100 mg) were homogenized in 2:1:0.8 chloroform:methanol:PBS mixture and then centrifuged. The organic layer was removed and triglyceride content was measured by the enzymatic method using a commercial kit (#OSR60118, Beckman Coulter, Inc., Brea, US).
Coelho M.S., de Lima C.L., Royer C., Silva J.B., Oliveira F.C., Christ C.G., Pereira S.A., Bao S.N., Lima M.C., Pitta M.G., Pitta I.R., Neves F.A, & Amato A.A. (2016). GQ-16, a TZD-Derived Partial PPARγ Agonist, Induces the Expression of Thermogenesis-Related Genes in Brown Fat and Visceral White Fat and Decreases Visceral Adiposity in Obese and Hyperglycemic Mice. PLoS ONE, 11(5), e0154310.
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3

Serum Lipid Profiling in Mice

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Body weight was measured at the end of the special diet, before the mice were euthanized at the beginning of an experiment. Serum was obtained through a cardiac puncture after 24 h of starvation, centrifuged at 12,500 rpm at 4 °C, and frozen at −80 °C until measured. Total cholesterol (Beckman Coulter, OSR6116, Brea, CA, USA), triglycerides (Beckman Coulter, OSR60118), HDL (Beckman Coulter, OSR6195), and LDL (Beckman Coulter, OSR6196) were measured with an AU chemistry analyzer (Beckman Coulter, AU480) according to the protocol of the manufacturer.
Christ C., Ocskay Z., Kovács G, & Jakus Z. (2024). Characterization of Atherosclerotic Mice Reveals a Sex-Dependent Susceptibility to Plaque Calcification but No Major Changes in the Lymphatics in the Arterial Wall. International Journal of Molecular Sciences, 25(7), 4046.
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4

Comprehensive Serum Biomarker Analysis

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Serum CHOL, TG, HDL, LDL, urea (OSR60118, OSR6116, OSR6187, OSR6183, OSR6234, Beckman Coulter Inc., Georgia, USA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) (C010-2–1, C009-2–1, Nanjing Jiancheng Bioengineering Institute, China) were measured using commercial enzymatic kits on a Beckman AU480 chemistry analyzer (Beckman Coulter Inc., Atlanta, GA, USA).
Zhang Y., Wang Y., Liao X., Liu T., Yang F., Yang K., Zhou Z., Fu Y., Fu T., Sysa A., Chen X., Shen Y., Lyu J, & Zhao Q. (2024). Glutamine prevents high-fat diet-induced hepatic lipid accumulation in mice by modulating lipolysis and oxidative stress. Nutrition & Metabolism, 21, 12.
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5

Plasma Insulin and Triglyceride Analysis

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Plasma was collected in heparin and frozen at -20°C until required. Plasma insulin concentration was determined by radioimmunoassay (RIA) (Sensitive Rat Insulin RIA kit, SRI-13K, Merck Millipore). Plasma triglyceride concentration was determined using an automated analyser (AU480, Beckman Coulter) and triglyceride reagent (OSR60118, Beckman Coulter).
Johnson H.M., Stanfield E., Campbell G.J., Eberl E.E., Cooney G.J, & Bell-Anderson K.S. (2019). Glucose mediates insulin sensitivity via a hepatoportal mechanism in high-fat-fed rats. The Journal of endocrinology, 241(3).
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