Sediment moisture content was determined by oven drying <2 mm sieved sediment at 105 °C for 24 h. Organic matter content was measured using loss-on-ignition in a muffle furnace (450 °C, 16 h)32 (link). Oven dried, root free sediment was analysed for C and N content using a TruSpec® analyzer (Leco Corp., St Joseph, MI, USA). Sediment samples were collected and shipped to Yara (Lincolnshire, UK) for texture analysis (Sand %, Silt %, Clay %) using a Mastersizer 3000 laser particle size analyzer (Malvern Panalytical). River water DOC and total dissolved N (TDN) content were determined using Multi N/C 2100 S analyser (AnalytikJena, Jena, Germany). The following chemical parameters were determined using river water samples and 0.5 M K2SO4 sediment extracts: concentrations of NH4+ and NO3− were measured according to the methods outlined by Mulvaney33 and Miranda34 (link) respectively. Total free amino acids and total free carbohydrates were determined using the fluorometric OPAME procedure35 (link) and the Myklestad method36 (link) respectively. The concentration of phenolic compounds was measured using the Folin-Ciocalteu method37 (link). Finally, molybdate-reactive P was measured for river water samples and 1 M 1.0 M CH3COOH sediment extracts38 (link).
Multi n c 2100s analyser
Manufactured by Analytik Jena
Sourced in Germany
The Multi N/C 2100S analyser is a high-performance instrument designed for the determination of total carbon (TC), total organic carbon (TOC), and total nitrogen (TN) in a variety of samples. The analyser utilizes state-of-the-art technology to provide accurate and reliable results.
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Lab products found in correlation
5 protocols using multi n c 2100s analyser
1
Comprehensive Sediment and Water Analysis
2
Total Organic Carbon Measurement
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Total organic carbon (TOC) measurements were performed on a multi N/C 2100 S analyser (Analytik Jena AG, Jena, Germany). The carbon concentration in the samples was determined as non-purgeable organic carbon (NPOC) with triplicate injection (100 μL). The analysed samples of the supernatants and the stock and purified suspensions were measured undiluted in triplicates.
3
Urine Composition Preservation Methods
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A mixed subsample of the collected urine was used to determine the best method of urine storage to preserve composition. Unfiltered and filtered (0.45 µm, vacuum filtration) subsamples (18 experimental units in total) were stored at ambient laboratory temperature (room temperature), refrigerated (8 °C) or frozen (− 20 °C) and analysed for total N, NH4+-N and NO3–N on the day of collection and after 7, 14, 28 and 49 days. Total organic N was estimated by deducting inorganic N (NH4+-N and NO3–N) from total N.
Following dilution (1000-fold) with ultrapure water, total urine N was determined using a Multi N/C 2100S analyser (AnalytikJena, Jena, Germany). Urine NH4+-N (urine diluted tenfold with ultrapure water) and NO3–N (undiluted urine) were determined by colorimetric reactions and spectrophotometry using the methods of Mulvaney et al.52 and Miranda et al.53 (link), respectively.
Following dilution (1000-fold) with ultrapure water, total urine N was determined using a Multi N/C 2100S analyser (AnalytikJena, Jena, Germany). Urine NH4+-N (urine diluted tenfold with ultrapure water) and NO3–N (undiluted urine) were determined by colorimetric reactions and spectrophotometry using the methods of Mulvaney et al.52 and Miranda et al.53 (link), respectively.
4
Soil Microbial Biomass Determination
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Soil microbial biomass C (MBC) and N (MBN) were determined by fumigation extraction (Brookes et al. 1985; Vance et al. 1987 ) at day 7 (vial A) and day 42 (vial B) of incubation. To reduce the amount of inorganic N in the sample, 15 g moist soil were preextracted for 30 min by oscillating shaking at 200 rev min -1 with 40 ml 0.05 M K 2 SO 4 (Widmer et al. 1989 ). Non-fumigated and fumigated 5-g portions were extracted for 30 min by oscillation shaking at 200 rev min -1 with 20 ml 0.05 M K 2 SO 4 (Potthoff et al. 2003) , centrifuged (3000 g for 10 min at 10 °C), filtered (hw3, Sartorius Stedim Biotech, Göttingen, Germany), and stored at -18 °C before analysis. Organic C and total N in the extracts were determined with a Multi N/C 2100S analyser (Analytik Jena, Germany). MBC was calculated as E C /k EC , where E C = (organic C extracted from fumigated soils) -(organic C extracted from non-fumigated soils) and k EC = 0.45 (Wu et al. 1990) . MBN was calculated as E N /k EN , where E N = (total N extracted from fumigated soils) -(total N extracted from nonfumigated soils) and k EN = 0.54 (Brookes et al. 1985) . About 14 ml of the soil extracts were freeze-dried for isotope analysis (Alpha 1-4 LD plus, Christ, Osterode, Germany).
5
Soil Microbial Biomass Determination
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Soil microbial biomass C (MBC) and N (MBN) were determined by fumigation extraction (Brookes et al. 1985; Vance et al. 1987 ) at day 7 (vial A) and day 42 (vial B) of incubation. To reduce the amount of inorganic N in the sample, 15 g moist soil were preextracted for 30 min by oscillating shaking at 200 rev min -1 with 40 ml 0.05 M K 2 SO 4 (Widmer et al. 1989 ). Non-fumigated and fumigated 5-g portions were extracted for 30 min by oscillation shaking at 200 rev min -1 with 20 ml 0.05 M K 2 SO 4 (Potthoff et al. 2003) , centrifuged (3000 g for 10 min at 10 °C), filtered (hw3, Sartorius Stedim Biotech, Göttingen, Germany), and stored at -18 °C before analysis. Organic C and total N in the extracts were determined with a Multi N/C 2100S analyser (Analytik Jena, Germany). MBC was calculated as E C /k EC , where E C = (organic C extracted from fumigated soils) -(organic C extracted from non-fumigated soils) and k EC = 0.45 (Wu et al. 1990) . MBN was calculated as E N /k EN , where E N = (total N extracted from fumigated soils) -(total N extracted from nonfumigated soils) and k EN = 0.54 (Brookes et al. 1985) . About 14 ml of the soil extracts were freeze-dried for isotope analysis (Alpha 1-4 LD plus, Christ, Osterode, Germany).
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