Primary antibodies including anti-mouse CD8a-PE (BioLegend, 100707, 53–6.7, 1:100), anti-mouse CD45-PE (BD Pharmingen,561087, 30-F11, 1:100), anti-mouse CD45.1-FITC (BioLegend, 110705, A20, 1:100), anti-mouse CD45.2-PE (BioLegend, 109807,104, 1:100), anti-human CD45-PE (BD Pharmingen, 555483, HI30, 1:100), anti-human CD33-APC (Biolegend,366605, P67.6, 1:100), anti-human CD3-FITC (BioLegend, 300305, HIT3a, 1:100), anti-human CD8-PE (BD Pharmingen, 555367, RPA-T8, 1:100) antibodies were used. Cells were run on Calibur for analysis or FACSAria for sorting.
Anti mouse cd45.1 fitc
Manufactured by BioLegend
Sourced in United States
Anti-mouse CD45.1-FITC is a fluorochrome-conjugated antibody that binds to the CD45.1 allotype of the mouse CD45 protein. CD45 is a transmembrane protein tyrosine phosphatase that is expressed on the surface of most hematopoietic cells.
Automatically generated - may contain errors
Lab products found in correlation
Anti human cd3 fitc, by BioLegend (2 mentions)
Pe anti mouse cd45, by BD (2 mentions)
Pe anti mouse cd8a, by BioLegend (2 mentions)
Anti mouse cd45.2 pe, by BioLegend (2 mentions)
Apc anti human cd33, by BioLegend (2 mentions)
Anti human cd8 pe, by BD (2 mentions)
Anti human cd45 pe, by BD (2 mentions)
Deferasirox, by Novartis (1 mentions)
Fetal calf serum (fcs), by Sartorius (1 mentions)
Cd45.2 pe, by BioLegend (1 mentions)
Cd117 microbead, by Miltenyi Biotec (1 mentions)
Ter119, by Thermo Fisher Scientific (1 mentions)
Calcein am fluorescent dye, by Merck Group (1 mentions)
Gr1 pe cy7, by BioLegend (1 mentions)
Anti mouse sca1 pe, by Thermo Fisher Scientific (1 mentions)
Apc cy7 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)
Cd45r b220, by Thermo Fisher Scientific (1 mentions)
M3434 methylcellulose, by STEMCELL (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Cd11b, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mouse cd45.1 fitc
1
Multiparameter Flow Cytometry Analysis
2
Multicolor Flow Cytometric Analysis of Hematopoietic Cells
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Anti-mouse-CD45.1-FITC, CD45.2-PE, Gr-1-PE/CY7, CD45R/B220-PE/CY5.5 CD11b-PE/CY7 and CD3-APCwere purchased from BioLegend (San Diego, CA, USA); anti-mouse-Sca-1-PE, CD117 (c-kit) Alexa Fluor 700, CD4, CD8, CD45R/B220, Gr-1, CD11b, Ter119 and APC/CY7-conjugated streptavidin were purchased from eBioscience (San Diego, CA, USA); iron-dextrin was purchased from Pharmacosmos A/S (Denmark); the ROS staining kit (S0033) and NAC were purchased from the Beyotime Institute of Biotechnology; calcein-AM fluorescent dye was purchased from Sigma-Aldrich (USA); deferasirox was purchased from Novartis; RPMI 1640 was purchased from Gibco (USA); methylcellulose M3434 was purchased from Stem cell (USA); fetal calf serum was purchased from Bioind (Italy); CD117 MicroBeads (130-091-229) were purchased from Miltenyi Biotec (Germany); a RNA PCR Kit (AMV) Ver.3.0 (DRR019A) was purchased from Takara; a RNeasy MicroKit (74004) was purchased from Qiagen (Germany); the NOX4 and GPX1 gene qRT-PCR primers were synthesized by Sangon Biotech (Shanghai, China).
3
Multiparameter Flow Cytometry Analysis
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