Eosin y

Manufactured by Fujifilm

Sourced in Japan, United States

Eosin Y is a fluorescent dye used in histological and cytological staining procedures. It is a water-soluble, bright pink-colored compound that is commonly used in combination with other dyes, such as hematoxylin, to stain biological samples for microscopic examination.

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Lab products found in correlation

Paraformaldehyde (pfa), by Fujifilm (4 mentions) Mayer s hematoxylin, by Fujifilm (3 mentions) Mayer s hematoxylin solution, by Fujifilm (3 mentions) Pbs pva, by Fujifilm (2 mentions) Hematoxylin, by Fujifilm (2 mentions) Axio imager z2, by Zeiss (2 mentions) Jb 4 resin, by Polysciences (2 mentions) Ethyl acetate, by Fujifilm (2 mentions) Acetonitrile, by Fujifilm (2 mentions) Perylene, by Fujifilm (2 mentions) Acridine yellow g, by Merck Group (1 mentions) Rhodamine 6g, by Fujifilm (1 mentions) Hematoxylin, by Muto Pure Chemicals (1 mentions) Haematoxylin, by Fujifilm (1 mentions) Microscope slide, by Matsunami (1 mentions) Modified mayer s hematoxylin, by Merck Group (1 mentions) Avidin biotin peroxidase complex, by Merck Group (1 mentions) Goat anti rabbit igg, by Merck Group (1 mentions) Mavs ko mice, by Jackson ImmunoResearch (1 mentions) Isoflurane, by Baxter (1 mentions)

Close spelling variants of this product

1% Eosin Y Solution (16 citations) 0.5% eosin Y ethanol solution (5 citations)

21 protocols using eosin y

Molecular Dye Incorporation in HEWL Crystals

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Molecular loading into the
HEWL crystals was performed using the following procedure. The sealing
film was initially peeled off from the crystallization plate, and
7 μL of various concentrations of the dye in a sodium acetate
buffer solution was added to the well containing HEWL crystals. Subsequently,
the plate was sealed again and placed under an inverted microscope
for optical measurement. Notably, HEWL was mixed with the dye solution
(50 mg/mL) to avoid crystal dissolution, owing to decreasing concentration.
The dye types employed in this study are provided in Chart 1. Acridine yellow G was purchased
from Sigma-Aldrich, whereas eosin Y and rhodamine 6G were obtained
from Wako Chemical and used without further purification.

Uwada T., Kouno K, & Ishikawa M. (2020). In Situ Absorption and Fluorescence Microspectroscopy Investigation of the Molecular Incorporation Process into Single Nanoporous Protein Crystals. ACS Omega, 5(16), 9605-9613.

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Hematoxylin and Eosin Staining of Tumor-Associated Macrophages

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For H&E staining, M0, TAM(Ca), TAM(CAF) and TAM(TAM) were seeded in the Nunc Lab-Tek II Chamber Slide system (cat. no. 154526PK; Thermo Fisher Scientific, Inc.). The subsequent steps were all performed at room temperature. The slides were gently washed twice with PBS and fixed with 90% ethanol for 15 min. Then, the slides were stained with hematoxylin (Muto Pure Chemicals Co., Ltd.) for 10 min, washed under running water for 5 min and stained with eosin Y (FUJIFILM Wako Pure Chemical Corporation) for 5 min. After washing under running water for 5 min to remove the excess dye, the slides were sealed with a coverslip and neutral resin. Differences between each group were compared under a light microscope (magnification, ×200).

Chen S., Morine Y., Tokuda K., Yamada S., Saito Y., Nishi M., Ikemoto T, & Shimada M. (2021). Cancer-associated fibroblast-induced M2-polarized macrophages promote hepatocellular carcinoma progression via the plasminogen activator inhibitor-1 pathway. International Journal of Oncology, 59(2), 59.

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Hematoxylin and Eosin Staining of Paraffin Sections

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Hematoxylin and eosin staining was performed using paraffin embedded sections. After the sections were deparaffinized, specimens were stained with Mayer’s hematoxylin (Muto, Tokyo, Japan) for 3 min. After being rinsed in distilled water, the specimens were stained with 1% eosin Y (Wako, Osaka, Japan) for 1 min, followed by dehydration with 99.5% ethanol.

Kamaguchi M., Iwata H., Ujiie I., Ujiie H., Sato J., Kitagawa Y, & Shimizu H. (2018). Direct Immunofluorescence Using Non-Lesional Buccal Mucosa in Mucous Membrane Pemphigoid. Frontiers in Medicine, 5, 20.

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Isolation and Characterization of Spheroids

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To isolate spheroids from MC medium, 0.5 volume of cellulose solution (Yakult Pharmaceutical Industry, adjusted to 5 U/ml with normal culture medium) relative to the volume of MC medium was added and incubated for 30 minutes at 37 °C to reduce the viscosity of the MC medium. Isolated spheroids were fixed with 4% paraformaldehyde (PFA, Wako) for 15 minutes at room temperature. Spheroids embedded in paraffin were sectioned at a thickness of 8 µm by a microtome. Sections were stained with haematoxylin (Wako) and eosin Y (Wako).

Tao F., Sayo K., Sugimoto K., Aoki S, & Kojima N. (2020). Development of a tunable method to generate various three-dimensional microstructures by replenishing macromolecules such as extracellular matrix components and polysaccharides. Scientific Reports, 10, 6567.

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Histological Analysis of Tissue Samples

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Histological analysis was performed as described in previous studies (48 (link), 49 (link)) with some modifications. Briefly, tissues were excised from each mouse and fixed in 4% (v/v) paraformaldehyde/PBS. After ethanol dehydration, the fixed samples were embedded in paraffin, cut into 5 μm sections with a microtome, and mounted on microscope slides (Matsunami Glass). The sections were stained with modified Mayer’s hematoxylin (Merck) and eosin Y (Wako Pure Chemical Industries Ltd). For immunohistochemistry analysis, the sections were incubated in 1% (v/v) hydrogen peroxide in methanol and treated with 10% (v/v) normal goat serum, rabbit anti-UCP1 (U6382; 1:200; Sigma–Aldrich), goat anti-rabbit IgG (Nichirei), and avidin-biotin-peroxidase complex (Nichirei).

Takahashi H., Tokura M., Kawarasaki S., Nagai H., Iwase M., Nishitani K., Okaze H., Mohri S., Ito T., Ara T., Jheng H.F., Nomura W., Kawada T., Inoue K, & Goto T. (2022). Metabolomics reveals inosine 5′-monophosphate is increased during mice adipocyte browning. The Journal of Biological Chemistry, 298(10), 102456.

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Influenza Virus Infection in MAVS KO Mice

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MAVS KO mice (#008634) were obtained from Jackson Laboratory. C57BL/6J mice were obtained from Crea Japan. Mice were pretreated with 30 μL of THGP (50 or 100 mg/kg) by i.n. injection at 3 h before infection. Mice were infected with IAV (A/Puerto Rico/8/1934 H1N1 strain, 1 × 10⁵ pfu/mice; i.n.). During the treatment mice were anesthetized with isoflurane (Baxter). At the indicated time after infection, the lung tissues were used for further experiments. These animal experiments were approved by the committee reviews of Hokkaido University (No. 19-0041). For Hematoxylin-Eosin Stain, formalin-fixed paraffin-embedded lung tissues were stained with Mayer’s hematoxylin (Wako) and 1% Eosin Y (Wako) following the manufacturer’s protocol.

Baidya S., Nishimoto Y., Sato S., Shimada Y., Sakurai N., Nonaka H., Noguchi K., Kido M., Tadano S., Ishikawa K., Li K., Okubo A., Yamada T., Orba Y., Sasaki M., Sawa H., Miyamoto H., Takada A., Nakamura T, & Takaoka A. (2021). Dual Effect of Organogermanium Compound THGP on RIG-I-Mediated Viral Sensing and Viral Replication during Influenza a Virus Infection. Viruses, 13(9), 1674.

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Synthesis and Characterization of Polymeric Microspheres

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2-Methoxyethyl acrylate (MEA, purity 98%),
styrene (St, 99%), butyl acrylate (BA, 99%), potassium peroxodisulfate
(KPS, 95%), sodium dodecyl sulfate (SDS, 95%), disodium hydrogenphosphate
(99%), eosin Y (EoY, 95%), phloxine B (PhB, 98%), erythrosine (Ery,
95%), rose bengal (RB, 95%), orange II (OrII, 98%), tartrazine (Ttz,
98%), and ethanol (EtOH, 99.5%) were purchased from Wako Pure Chemical
Industries and used as received. 2-(2-Ethoxyethoxy) ethyl acrylate
(ET2A, 98%), 4-methoxystyrene (MSt, 98%), N-isopropylacrylamide
(NIPAm, 98%), and N,N′-methylenebis(acrylamide)
(BIS, 97%) were purchased from Tokyo Chemical Industry and used as
received. 2-(2-Methoxyetoxy) ethyl acrylate (ME2A, 95%) was purchased
from Monomer-Polymer and Dajac Labs, Inc. 2-[2-(2-Methoxyethoxy)ethoxy]ethyl
acrylate (Me3A, 98%) was kindly donated by Kyoeisha Chemical Co.,
Ltd. The cross-linker, ethylene glycol dimethacrylate (EGDMA, 98%),
was purchased from Sigma-Aldrich and used as received. Water used
for microsphere preparations was distilled and then ion-exchanged
(EYELA, SA-2100E1).

Kureha T., Nishizawa Y, & Suzuki D. (2017). Controlled Separation and Release of Organoiodine Compounds Using Poly(2-methoxyethyl acrylate)-Analogue Microspheres. ACS Omega, 2(11), 7686-7694.

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Hematoxylin and Eosin Staining of Cell Complexes

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Next, the complexes were subjected to hematoxylin and eosin Y staining. The media were carefully removed from the 96-well plates after five days of incubation and the complexes were washed
three times with PBS-PVA and fixed in 4% (w/v) paraformaldehyde (Wako Pure Chemical Industries, Osaka, Japan) for 30 min. The complexes were then washed three additional times with PBS-PVA.
Mayer’s hematoxylin solution (Wako Pure Chemical Industries) was used to stain the complexes for 10 min, then washed out with tap water for 10 min. Finally, the complexes were stained with
eosin Y (1%; Wako Pure Chemical Industries) for 2 min, and the eosin Y was washed out with tap water for one minute. A glycerol-water mixture (1:4) was used to mount the complexes.

ALAM M.H., LEE J, & MIYANO T. (2018). GDF9 and BMP15 induce development of antrum-like structures by bovine granulosa cells without oocytes. The Journal of Reproduction and Development, 64(5), 423-431.

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Histological Analysis of Subcutaneous Tumors

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Resected subcutaneous tumours from tumour-bearing mice were fixed with 10% neutral buffered formalin then embedded in paraffin. The paraffin blocks were sliced to a thickness of 5 μm, after which the paraffin-embedded sections were deparaffinised and stained with Mayer’s haematoxylin solution (Wako) and eosin Y (Wako) for histopathological analysis.

Ogawa S., Kubo H., Murayama Y., Kubota T., Yubakami M., Matsumoto T., Yamamoto Y., Morimura R., Ikoma H., Okamoto K., Kamiya M., Urano Y, & Otsuji E. (2021). Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal. Scientific Reports, 11, 17946.

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Histological Analysis of Gastric Tissues

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Gastric tissues were fixed using 4% paraformaldehyde in phosphate buffer solution (Wako Pure Chemical Industries) at 4 °C overnight. Next, these tissues were immersed in 15% sucrose in PBS at 4 °C overnight, frozen in dry ice powder, and sliced into 10-μm-thick sections. Sections got fully dipped with Mayer’s hematoxylin (Wako Pure Chemical Industries) and eosin Y (Wako Pure Chemical industries), sequentially. The HE stained specimens were observed with a light field microscopy (BZ-X710; Keyence).

Yang C., Sugimoto K., Murata Y., Hirata Y., Kamakura Y., Koyama Y., Miyashita Y., Nakama K., Higashisaka K., Harada K., Katada R, & Matsumoto H. (2020). Molecular mechanisms of Wischnewski spot development on gastric mucosa in fatal hypothermia: an experimental study in rats. Scientific Reports, 10, 1877.

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