Injectable preparations of phenobarbital sodium were provided from Exir Pharmaceutical Co (Iran). Glacial acetic acid, ethanol (99.9%), diethyl-ether, ethylenediaminetetraacetic acid (EDTA), H2O2, potassium dihydrogen phosphate (KH2PO4), di-potasium hydrogen phosphate (K2HPO4), and sodium chloride were all purchased from Merck™ (Darmstadt, Germany). BDS as the active pharmaceutical ingredient was supplied from Farmabios (Pavia, Italy). Normal saline solution (NaCl 0.9% w/v) was prepared in-house. Formaldehyde was obtained from Mojalali Company (Tehran, Iran). Hexadecyltrimethyl ammonium bromide (HETAB) and O-dianisidine dihydrochloride were purchased from Sigma-Aldrich® (United States). The rat TNF-α ELISA kit was supplied from Bender MedSystem™ (Vienna, Austria). In this study, doubled-distilled water which was previously filtered through membrane filters (0.45 μm) was used in all cases. Self-assembled hyaluronic acid nanoparticles containing BDS were prepared, characterized, and reported previously by the authors [30 (link)]. All other reagents were of pharmaceutical or analytical grade and were used as received.
Hexadecyltrimethyl ammonium bromide hetab
Manufactured by Merck Group
Sourced in United States
Hexadecyltrimethyl ammonium bromide (HETAB) is a cationic surfactant. It is a white crystalline powder that is soluble in water and organic solvents. HETAB is commonly used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
O dianisidine dihydrochloride, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
Hydrogen peroxidase, by Merck Group (1 mentions)
Urethane, by Merck Group (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
O dianisidine, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Griess reagent, by Merck Group (1 mentions)
3 protocols using hexadecyltrimethyl ammonium bromide hetab
1
Formulation and Characterization of BDS Nanoparticles
2
Anti-Inflammatory Protocol Using EIQ and EIE
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EIQ was kindly supplied by Prof. Whang (Herb Medicine Laboratory, Chung-Ang University, Seoul, Korea) and was dissolved in distilled water for animal treatments. 2,4,6-Trinitrobenzene sulfonic acid (TNBS), sodium carboxymethylcellulose (CMC-Na), 5-aminosalicylic acid (5-AS), EDTA, EGTA, urethane, hexadecyl trimethyl ammonium bromide (HETAB), hydrogen peroxidase, dimethylformamide were purchased from Sigma (St.Louis, MO, USA). EIE was kindly provided by Dong-A Pharmaceutical Co. Ltd (Yong-In, Korea) and was dissolved in CMC-Na for animal treatments. TNF-α and NO assay kits were purchased from Cayman Chemical (Ann Arbor, MI, USA).
3
Oxidative Stress Biomarker Quantification
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Ethylene diamine tetra acetic acid, epinephrine bitartarate, hydrogen peroxide, trichloroacetic acid, 5,5-dithiobis (2-nitrobenzoic acid), thiobarbituric acid, Griess reagent, hexadecyl trimethyl ammonium bromide (HETAB) and O-dianisidine were procured from Sigma Aldrich, India. Calcium and total protein assay kits were procured from Autospan Diagnostics Ltd., India.
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