2 apb

Thapsigargin (Sigma, T9033), Tunicamycin (Sigma, T7765), Maprotiline (Sigma, M9651), Amoxapine (MedChemExpress, HY-B0991), Desloratadine (MedChemExpress, HY-B0539), Desipramine (Sigma, D3900), Trifluoperazine (Sigma, T8516), Clomipramine (Sigma, C7291), Amitriptyline (Sigma, A8404), Quetiapine (Sigma, Q3638), Olanzapine (MedChemExpress, HY-14541), Doxepin (MedChemExpress, B078), Loxapine (Sigma, L106), NorQuetiapine (Sigma, 07849), dimethyl sulfoxide (DMSO) (Sigma, D8418), 2-APB (MedChemExpress, HYW009724), U-73122 (MedChemExpress, HY13419), Go 6983 (MedChemExpress, HY13689), GSK2606414 (Sigma, 516535), ISRIB (MedChemExpress, HY-12495).

Human-derived THP-1 cells (ATCC^® TIB-202) were cultured in RPMI-1640 medium containing 10% heat-iNACtivated fetal bovine serum and 1% penicillin/streptomycin in a humidified incubator with 5% CO₂ at 37°C. Cells were treated with phorbol myristate acetate (PMA, 100 nM) for 48 h to induce macrophage differentiation. In transfection experiment, cells were transfected with 50 nM small interfering RNA (siRNA) targeting STIM1 (5′-GTGGTACAGTGGCTGATCA-3′) or negative control sequence (RiboBio, Guangzhou, China) using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, United States) according to the manufacturer’s instructions. In pharmacological experiment, cells were pretreated with 3 mM NAC (Sigma-Aldrich, St. Louis, MO, United States) for 1 h, with 10 μM SKF-96365 (MedChemExpress, United States) for 2 h, or with 10 μM 2-APB (MedChemExpress, United States) for 2 h before CSE stimulation.

All salts were from Sangon Biotech in Shanghai. Acetylcholine, N^ω-nitro-L-arginine and indomethacin were offered by Sigma-Aldrich (St Louis, USA). NE was purchased from GRANDPHARMA (China) co. LTD. 17β-Estradiol, LPS, Propyl pyrazole triol (PPT), MPP hydrochloride (MPP), G-1, G-15, Diarylpropionitrile (DPN), PHTPP, ouabain, TRAM-34, apamin, U73122, U73343, 2-APB and SKA-31 were supplied by MedChemExpress (MCE; New Jersey, USA), SN-6 was provided by Tocris (Bristol, UK). LiCl was supplied by Macklin (China). The human phospholipase C Beta 1 Polyclonal antibody (Cat No. 26551-1-AP) and human ITPR1-specific Polyclonal antibody (Cat No. 19962-1-AP) were from proteintech. The commercial kits of serum ALT, AST, LD and BUN were from Neobioscience (Jiancheng Bioengineering Institute, Nanjing, China). The commercial kit of serum DAO was from Solarbio. The commercial kit of serum Cr was from Beijing Leagene Biotechnology co. LTD.