Tlc sheets silica 60 f254

Manufactured by Merck Group

Sourced in Germany

TLC sheets silica 60 F254 are thin-layer chromatography (TLC) plates designed for analytical and preparative chromatographic separations. The plates are coated with a silica gel 60 adsorbent material that contains a fluorescent indicator F254, which allows for visualization of separated compounds under ultraviolet (UV) light.

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Lab products found in correlation

Silica gel 60n, by Kanto Chemical (3 mentions) Jms dx303hf, by JEOL (3 mentions) Avance 600, by Bruker (3 mentions) Ft ir 410, by Jasco (1 mentions) Jms dx303, by JEOL (1 mentions)

4 protocols using tlc sheets silica 60 f254

Efficient Fume Hood Synthesis Protocol

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All reactions were performed in an efficient fume hood. Chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA), Honeywell Fluka (Morristown, NJ, USA), Kanto Chemical (Tokyo, Japan), Nacalai Tesque (Kyoto, Japan), Tokyo Chemical Industry (Tokyo, Japan) and FUJIFILM Wako (Osaka, Japan). Commercially available reagent-grade chemicals were used without further purification. The reaction progress was monitored by a thin layer chromatography (TLC) on precoated plates [Merck (Darmstadt, Germany) TLC sheets silica 60 F254]. The flash column chromatography was carried out on Silica Gel 60N [40–100 mesh, Kanto Chemical (Tokyo, Japan)]. Melting points were determined on a Yanaco (Kyoto, Japan) melting point apparatus and were uncorrected. ¹H and ¹³C NMR spectra were registered on a Bruker (Billerica, MA, USA) Avance 600 (600 MHz). Chemical shits are referenced to TMS. Mass spectra (MS) and high-resolution mass spectra (HRMS) were recorded on a JEOL (Tokyo, Japan) JMS-DX303HF using positive fast atom bombardment (FAB) with 3-nitrobenzyl alcohol as the matrix.

I. Ciftci H., O. Radwan M., E. Ozturk S., Ulusoy N.G., Sozer E., E. Ellakwa D., Ocak Z., Can M., F.S. Ali T., I. Abd-Alla H., Yayli N., Tateishi H., Otsuka M, & Fujita M. (2019). Design, Synthesis and Biological Evaluation of Pentacyclic Triterpene Derivatives: Optimization of Anti-ABL Kinase Activity. Molecules, 24(19), 3535.

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General Synthetic Procedure and Characterization

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General Procedure Pertaining to Synthesis All reagents were commercially available with high purity grade. TLC was performed on precoated plates, TLC sheets silica 60 F254 (Merck, Darmstadt, Germany) or TLC sheets Chromatorex NH silica (Fuji Silysia Chemical, Kasugai, Japan). Chromatography was carried out on Silica Gel 60N (40-100 mesh) (Kanto Chemical, Tokyo, Japan) and NH silica gel Chromatorex (NH, 100-200 mesh) (Fuji Silysia Chemical). NMR spectra were recorded on a JEOL (Tokyo, Japan) JNM-AL300 (300 MHz) and Bruker (Billerica, MA, U.S.A.) Avance 600 (600 MHz). Chemical shifts were referenced to tetramethylsilane (TMS). Mass spectrum (FAB) and high-resolution mass spectra (HRMS) were recorded by a JEOL JMS-DX303. HRMS were recorded by using positive fast atom bombardment (FAB) with 3-nitrobenzyl alcohol (NBA) as the matrix. IR-red spectra were recorded on a JASCO (Tokyo, Japan) FT/IR-410. The samples were prepared as KBr discs or thin films between sodium chloride discs. Melting points were determined on a Yanaco (Kyoto, Japan) melting point apparatus and were uncorrected.

Tateishi H., Tateishi M., Radwan M.O., Masunaga T., Kawatashiro K., Oba Y., Oyama M., Inoue-Kitahashi N., Fujita M., Okamoto Y, & Otsuka M. (2021). A New Inhibitor of ADAM17 Composed of a Zinc-Binding Dithiol Moiety and a Specificity Pocket-Binding Appendage. Chemical & pharmaceutical bulletin, 69(11).

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Characterization of Organic Compounds

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All chemical compounds were purchased from Merch (Tokyo, Japan), Alfa Aesar (Ward Hill, MA, USA), Tokyo Chemical Industry (Tokyo, Japan), Kanto Chemical (Tokyo, Japan), Nacalai Tesque (Kyoto, Japan) and FUJFILM-Wako (Osaka, Japan). Thin-layer chromatography (TLC) was performed to follow the progress of the reactions on pre-coated plates (Merck TLC sheets silica 60 F254). NMR spectra (BRUKER 600 MHz) used TMS as a reference. A JEOL JMSDX303HF recorded mass spectra (EI), and high-resolution mass spectra (HRMS). HRMS were recorded using positive fast atom bombardment (FAB) with 3-nitrobenzyl alcohol (NBA) as the matrix.

Ali T.F., Ciftci H.I., Radwan M.O., Roshdy E., Shawky A.M., Abourehab M.A., Tateishi H., Otsuka M, & Fujita M. (2022). Discovery of Azaindolin-2-One as a Dual Inhibitor of GSK3β and Tau Aggregation with Potential Neuroprotective Activity. Pharmaceuticals, 15(4), 426.

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Analytical Characterization of Compounds

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General Procedure Pertaining to Synthesis TLC was performed on precoated plate [Merck (Darmstadt, Germany) TLC sheets silica 60 F 254 ]. Chromatography was carried out on Silica Gel 60N (40-100 mesh) (Kanto Chemical, Tokyo, Japan). NMR spectra were recorded on a Bruker (Billerica, MA, U.S.A.) Avance 600 (600 MHz). Chemical shifts were referenced to tetramethylsilane (TMS). Melting points were determined on a Yanaco (Kyoto, Japan) Melting Point Apparatus. High-resolution mass spectra (HRMS) were recorded by a JEOL (Tokyo, Japan) JMS-DX303HF using positive fast atom bombardment (FAB) with 3-nitrobenzyl alcohol (NBA) as the matrix.

Nakagawa R., Tateishi H., Radwan M.O., Chinen T., Ciftci H., Iwamaru K., Baba N., Tominaga Y., Koga R., Toma T., Inoue J.I., Umezawa K., Fujita M, & Otsuka M. (2022). A New 1,2-Naphthoquinone Derivative with Anti-lung Cancer Activity. Chemical & pharmaceutical bulletin, 70(7).

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