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C1 ifc microfluidic chips

Manufactured by Standard BioTools
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The C1 IFC microfluidic chips are a core product from Standard BioTools. They are designed to enable single-cell analysis by providing a platform for the isolation, processing, and analysis of individual cells. The chips facilitate the capture, lysis, and reverse transcription of single cells, enabling downstream molecular profiling and characterization.

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Lab products found in correlation

C1 dna seq cell wash buffer, by Standard BioTools (2 mentions) Open app program, by Standard BioTools (2 mentions) C1 script builder software, by Standard BioTools (2 mentions) Ctr 6000 microscope, by Leica (2 mentions) C1 single cell auto prep system, by Standard BioTools (2 mentions)

Close spelling variants of this product

IFC) chip C1 chip

2 protocols using c1 ifc microfluidic chips

1

Single-Cell T-ATAC-Seq Using Fluidigm C1

Check if the same lab product or an alternative is used in the 5 most similar protocols
We adapted the C1 Single-Cell Auto Prep System with its Open App™ program (Fluidigm, Inc.) to perform T-ATAC-seq. Single T cells were captured using the C1 IFC microfluidic chips (small; 5–10micron) and custom-built T-ATAC-seq scripts generated using the C1™ Script Builder Software (scripts available from Fluidigm and upon request). Jurkat cells or peripheral blood T cells were first isolated by FACS sorting and then washed three times in C1 DNA Seq Cell Wash Buffer (Fluidigm). Cells were resuspended in DNA Seq Cell Wash Buffer at a concentration of 300 cells/μL and mixed with C1 Cell Suspension Reagent at a ratio of 3:2. 15μL of this cell mix was loaded onto the IFC. After cell loading, captured cells were visualized by imaging on a Leica CTR 6000 microscope.
Satpathy A.T., Saligrama N., Buenrostro J.D., Wei Y., Wu B., Rubin A.J., Granja J.M., Lareau C.A., Li R., Qi Y., Parker K.R., Mumbach M.R., Serratelli W.S., Gennert D.G., Schep A.N., Corces M.R., Khodadoust M.S., Kim Y.H., Khavari P.A., Greenleaf W.J., Davis M.M, & Chang H.Y. (2018). Transcript-indexed ATAC-seq for precision immune profiling. Nature medicine, 24(5), 580-590.
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2

Single-Cell T-ATAC-Seq Using Fluidigm C1

Check if the same lab product or an alternative is used in the 5 most similar protocols
We adapted the C1 Single-Cell Auto Prep System with its Open App™ program (Fluidigm, Inc.) to perform T-ATAC-seq. Single T cells were captured using the C1 IFC microfluidic chips (small; 5–10micron) and custom-built T-ATAC-seq scripts generated using the C1™ Script Builder Software (scripts available from Fluidigm and upon request). Jurkat cells or peripheral blood T cells were first isolated by FACS sorting and then washed three times in C1 DNA Seq Cell Wash Buffer (Fluidigm). Cells were resuspended in DNA Seq Cell Wash Buffer at a concentration of 300 cells/μL and mixed with C1 Cell Suspension Reagent at a ratio of 3:2. 15μL of this cell mix was loaded onto the IFC. After cell loading, captured cells were visualized by imaging on a Leica CTR 6000 microscope.
Satpathy A.T., Saligrama N., Buenrostro J.D., Wei Y., Wu B., Rubin A.J., Granja J.M., Lareau C.A., Li R., Qi Y., Parker K.R., Mumbach M.R., Serratelli W.S., Gennert D.G., Schep A.N., Corces M.R., Khodadoust M.S., Kim Y.H., Khavari P.A., Greenleaf W.J., Davis M.M, & Chang H.Y. (2018). Transcript-indexed ATAC-seq for precision immune profiling. Nature medicine, 24(5), 580-590.
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