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Quantitative enzyme linked immunosorbent assay kit

Manufactured by R&D Systems
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Sourced in United States

The Quantitative Enzyme-Linked Immunosorbent Assay (ELISA) kit is a laboratory tool used to measure the concentration of a specific protein or analyte in a sample. It utilizes antibodies and color change to quantify the target molecule. The kit provides the necessary reagents and protocols to perform the ELISA assay.

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Lab products found in correlation

Biotek synergy 4, by Agilent Technologies (1 mentions)

2 protocols using quantitative enzyme linked immunosorbent assay kit

1

Quantification of A549 Cell Secretions

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The supernatant from each A549 cell group was collected and a quantitative enzyme-linked immunosorbent assay kit (R&D Systems, Minneapolis, MN, USA) was used to analyze the A549 supernatant from each group, according to the manufacturer’s instructions. The supernatant of normal cultured A549 cells was used as the negative control.
Shen L., Zhang S.Q., Liu L., Sun Y., Wu Y.X., Xie L.P, & Liu J.C. (2017). Jolkinolide A and Jolkinolide B Inhibit Proliferation of A549 Cells and Activity of Human Umbilical Vein Endothelial Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 223-237.
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2

Cytokine Quantification in Mouse Sera

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The sera of mice in each treatment group were collected from the ophthalmic vein 7 days after treatments and assayed for mouse interferon-γ (IFN-γ), tumor necrosis factor α (TNF-α), and interleukin 12 (IL-12) levels using quantitative enzyme-linked immunosorbent assay kit (R&D systems), following validation of each ELISA according to the manufacturer's instructions. Absorbance was read using a Bio-Rad microplate reader (Biotek Synergy 4) at 488 nm.
Du C., Jiang J., Wan C., Pan G., Kong F., Zhai R., Hu C, & Ying H. (2022). AntiPD-L1 antibody conjugated Au-SPIOs nanoplatform for enhancing radiosensitivity and triggering anti-tumor immune response. Scientific Reports, 12, 19542.
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