Diagnosis tunel assay kit

U266 cells were treated with 30 μM of DDZ for 24 h, and MM1.S cells were pre-treated with 30 μM of DDZ for 3 h and treated with IL-6 (10 ng/mL) for 21 h. Late apoptotic cell death was determined with a Roche Diagnosis TUNEL assay kit, as described before [44 (link)].

Late apoptotic cell death was determined using a Roche Diagnosis TUNEL assay kit. SNU-1 and SNU-16 cells were treated with ABZ 50 µM for 24 h and fixed with 4% paraformaldehyde for 30 min and incubation with 0.2% triton X-100 for 10 min in fresh 1× PBS. Cells were washed by 1× PBS and incubated with TUNEL enzyme and TUNEL label for 1 h at 37 °C. Then cells were analyzed by BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences) [57 (link)].

Late apoptotic cell death was determined using a Roche Diagnosis TUNEL assay kit. PC-3 (5 × 10⁵ cells/well) and DU145 cells (5 × 10⁵ cells/well) were treated with EVO with or without HGF for the indicated times and at the indicated concentrations and then were fixed in 4% paraformaldehyde at room temperature for 30 min, washed with 1× PBS, and permeabilized with 0.2% Triton X-100 at room temperature for 15 min. The cells were then washed with 1× PBS and resuspended in TUNEL reaction mixture for 1 h at 37 °C in the dark. DNA damage in the stained cells was analyzed by a BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences, Becton-Dickinson, Franklin Lakes, NJ, USA) using the BD Accuri C6 Plus software [48 (link)].