Wide-field fluorescence and phase-contrast imaging were performed using a Nikon Ti2-E motorized inverted microscope controlled by the NIS Elements software with a SOLA 365 LED light source, a 100× objective lens (Oil CFI Plan Apochromat DM Lambda Series for Phase Contrast), and a Photometrics Prime 95B back-illuminated sCMOS camera or Hamamatsu Orca-Flash 4.0 LTS camera. Fusions to mNeonGreen (mNG) were imaged using a “YFP” filter set (C-FL YFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 500 ± 10 nm, Emission: 535 ± 15 nm, Dichroic Mirror: 515 nm). Fusions to msfGFP were imaged using a “GFP” filter set (C-FL GFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 436 ± 10 nm, Emission: 480 ± 20 nm, Dichroic Mirror: 455 nm). DAPI fluorescence was imaged using a “DAPI” filter set (C-FL DAPI, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 350 ± 25 nm, Emission: 460 ± 25 nm, Dichroic Mirror: 400 nm). mCherry was imaged using a “Texas Red” filter set (C-FL Texas Red, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 560 ± 20 nm, Emission: 630 ± 37.5 nm; Dichroic Mirror: 585 nm).
Orca flash 4.0 lts camera
Manufactured by Hamamatsu Photonics
The Orca-Flash 4.0 LTS camera is a scientific imaging device produced by Hamamatsu Photonics. It features a 4.2-megapixel CMOS image sensor and is capable of capturing images at high frame rates. The camera is designed for use in a variety of scientific and research applications.
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Lab products found in correlation
3 protocols using orca flash 4.0 lts camera
1
Multimodal Microscopy Imaging Protocol
2
Fluorescence and Phase-Contrast Imaging Setup
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Wide-field fluorescence and phase-contrast imaging were performed using a Nikon Ti2-E motorized inverted microscope controlled by the NIS Elements software with a SOLA 365 LED light source, a 100× objective lens (Oil CFI Plan Apochromat DM Lambda Series for Phase Contrast), and a Photometrics Prime 95B back-illuminated sCMOS camera or Hamamatsu Orca-Flash 4.0 LTS camera. Fusions to mNeonGreen (mNG) were imaged using a “YFP” filter set (C-FL YFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 500±10 nm, Emission: 535±15 nm, Dichroic Mirror: 515 nm). Fusions to msfGFP were imaged using a “GFP” filter set (C-FL GFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 436±10 nm, Emission: 480±20 nm, Dichroic Mirror: 455 nm). DAPI fluorescence was imaged using a “DAPI” filter set (C-FL DAPI, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 350±25 nm, Emission: 460±25 nm, Dichroic Mirror: 400 nm). mCherry was imaged using a “Texas Red” filter set (C-FL Texas Red, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 560±20 nm, Emission: 630±37.5 nm; Dichroic Mirror: 585 nm).
3
Multicolor Fluorescence Microscopy Imaging
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Wide-field fluorescence and phase-contrast imaging were performed using a Nikon Ti2-E motorized inverted microscope controlled by the NIS Elements software with a SOLA 365 LED light source, a 100 × objective lens (Oil CFI Plan Apochromat DM Lambda Series for Phase Contrast), and a Photometrics Prime 95B back-illuminated sCMOS camera or Hamamatsu Orca-Flash 4.0 LTS camera. Fusions to mNeonGreen (mNG) were imaged using a “YFP” filter set (C-FL YFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 500 ± 10 nm, Emission: 535 ± 15 nm, Dichroic Mirror: 515 nm). Fusions to msfGFP were imaged using a “GFP” filter set (C-FL GFP, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 436 ± 10 nm, Emission: 480 ± 20 nm, Dichroic Mirror: 455 nm). DAPI fluorescence was imaged using a “DAPI” filter set (C-FL DAPI, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 350 ± 25 nm, Emission: 460 ± 25 nm, Dichroic Mirror: 400 nm). mCherry was imaged using a “Texas Red” filter set (C-FL Texas Red, Hard Coat, High Signal-to-Noise, Zero Shift, Excitation: 560 ± 20 nm, Emission: 630 ± 37.5 nm; Dichroic Mirror: 585 nm).
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