After 24 hours culture, samples were prepared as described previously. In brief, cells were incubated with anti-integrin β1 antibody (Abcam, ab52971) overnight followed with the incubation of secondary anti-rabbit IgG antibody (Alexa Fluor 488 goat, Beyotime, Shanghai, China) and rhodamine phalloidin for 15 min at room temperature. Images were captured using a Zeiss LSM 710 microscope and analyzed by the CLSM image browser.
Alexa fluor 488 goat
Manufactured by Beyotime
Sourced in China, United States
Alexa Fluor 488 goat is a fluorescent dye conjugate used in various biomedical applications. It is a fluorescent label that can be attached to proteins, nucleic acids, and other biomolecules for detection and imaging purposes.
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Lab products found in correlation
2 protocols using alexa fluor 488 goat
1
Integrin β1 Expression Analysis
2
Immunofluorescence Analysis of Inflammatory Markers
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Cells were fixed in 4% formalin solution for 30 min and blocked with 5% bovine serum in 1 × PBS at 4 °C for 3 min. The primary antibodies rabbit anti-CD86 (1:200, Abcam, USA), rabbit anti-iNOS (1:200, Abcam, USA), rabbit anti–NF–κB p65 (1:200, Cell Signaling Technology, USA) and rabbit anti-phospho–NF–κB p65 (1:200, Cell Signaling Technology, USA) were applied to samples at 4 °C overnight and washed off thereafter. The samples were incubated with secondary antibodies (1:1000, Alexa Fluor 488 goat, Beyotime, USA) and FITC-phalloidin at 37 °C for 2 h. The cells were counterstained with DAPI and washed before imaging under an upright microscope.
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