Clinical parameters of each participants were obtained including diagnostic message, gender, age, BMI, and laboratory indexes performed on blood samples containing ALB (albumin), TG (total triglycerides), TC (total cholesterol), HDL-C (high density lipoprotein cholesterol), LDL-C (low density lipoprotein cholesterol), TB (total bilirubin), DB (direct bilirubin), TBA (total bile acid), BUN (blood urea nitrogen), Scr (serum creatinine), UmALB (urine microalbumin), Ucr (urine creatinine), and HbA1c (glycosylated hemoglobin) measured on AU5800 automatic analyzer (Beckman Coulter, CA, USA). Serum ACE levels were tested with appropriate Commercial kit (DEROM Biomedical Engineering Co., LTD, Hunan, China). In addition, eGFR (estimated glomerular filtration rate) was computed by modified MDRD equation and ACR (urinary microalbumin creatinine ratio) was also calculated as below: eGFR [ml.min-1. (1.73m2)] = 175*[Scr (mg/dl)]-1.154* (age)-0.203, female multiple with 0.742, ACR (mg/g) = UmALB * (113.1* Ucr) -1*106.
Au5800 automatic analyzer
Manufactured by Beckman Coulter
Sourced in United States
The AU5800 is an automatic analyzer designed for clinical chemistry and immunoassay testing. It features a high-throughput capacity and advanced automation capabilities to streamline laboratory workflows.
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Lab products found in correlation
7 protocols using au5800 automatic analyzer
1
Comprehensive Clinical Parameters Analysis
2
Metabolic Biomarkers in Chinese Adults
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All the subjects fasted overnight (at least 12 h). Blood samples were collected into 5 ml gel separator tubes (BD, USA) between 8 AM and 10 AM. Serum was subsequently isolated from the whole blood. Total cholesterol (CHO), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), fasting blood glucose (FBG), serum creatinine (CR), uric acid (UA) and homocysteine (HCY) were analyzed with a Beckman AU5800 automatic analyzer (Beckman Coulter, Tokyo, Japan).
The following diagnostic criteria were employed as recommended for Chinese adults [22 (link)]. Overweight and obesity were defined as 24.0 ≤ to ≤ 27.9 kg/m2 and body mass index (BMI) ≥ 28.0 kg/m2 respectively. Hyperuricemia was defined as serum UA level ≥ 420 μmol/l for males and ≥ 360 μmol/l for females. Hyperglycemia was defined as FBG > 6.90 mmol/l. Hyperhomocysteinemia was defined as HCY ≥ 10.0 μmol/l and high creatinine was defined as CR level > 115 μmol/l for males and > 107 μmol/l for females. Dyslipidemia was defined as TG ≥ 1.70 mmol/l, CHO ≥ 5.70 mmol/l, LDL-C ≥ 3.60 mmol/l, or HDL-C < 1.00 mmol/l [23 (link)].
The following diagnostic criteria were employed as recommended for Chinese adults [22 (link)]. Overweight and obesity were defined as 24.0 ≤ to ≤ 27.9 kg/m2 and body mass index (BMI) ≥ 28.0 kg/m2 respectively. Hyperuricemia was defined as serum UA level ≥ 420 μmol/l for males and ≥ 360 μmol/l for females. Hyperglycemia was defined as FBG > 6.90 mmol/l. Hyperhomocysteinemia was defined as HCY ≥ 10.0 μmol/l and high creatinine was defined as CR level > 115 μmol/l for males and > 107 μmol/l for females. Dyslipidemia was defined as TG ≥ 1.70 mmol/l, CHO ≥ 5.70 mmol/l, LDL-C ≥ 3.60 mmol/l, or HDL-C < 1.00 mmol/l [23 (link)].
3
Comprehensive Viral Hepatitis Profiling
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In total, 5 ml of blood samples was collected aseptically and centrifuged to separate the serum within 3 h, and all serum specimens were evaluated within 24 h. The HBsAg, hepatitis B surface antibody (HBsAb), hepatitis B pre‐core antigen (HBeAg), hepatitis B pre‐core antibody (HBeAb), and hepatitis B core antibody (HBcAb) levels were evaluated using the Abbott i2000sr automatic immunoassay analyzer (Abbott Company, the USA). The markers of liver and kidney function were determined using AU5800 Automatic Analyzer (Beckman Coulter, Galway, Ireland). Complete blood count was evaluated using BC6600 plus Analyzer (Beckman Coulter, Galway, Ireland).
4
Serum Biomarker Measurement Protocol
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Serum phosphate, calcium, alkaline phosphatase (ALP), and creatinine were measured by the Center of Laboratory Medicine, Fuwai Hospital. Briefly, phosphate was determined with a phosphate test kit (molybdate method) (Biosino, Beijing, China). Calcium was determined with a calcium test kit (o-Cresolphthalein complexone, OCPC method; Biosino, Beijing, China). Phosphate and calcium were measured using an AU 5800 automatic analyzer (Beckman Coulter, USA). ALP was determined with the ALP kit (NPP substrate-AMP buffer method; Biosino, Beijing, China). Creatine was determined with creatinine kit (Jaffé method; Biosino, Beijing, China). ALP and creatinine were measured using a Hitachi LABOSPECT 008AS automatic analyzer (Hitachi, Tokyo, Japan).
5
Liver Antioxidant Status Evaluation
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The levels of serum lipids such as TG and TC, as well as hepatotoxicity biochemical indicators such as LDL-C, alanine transaminase (ALT), aspartate aminotransferase (AST), and high-density lipoprotein cholesterol (HDL-C), were measured with an AU5800 automatic analyzer (Beckman Coulter, Inc., Brea, CA, USA).
The liver tissues were weighed according to the weight (g): volume (mL) = 1:9 and added to 9 times the volume of homogenate medium accurately. Then, the mixture was homogenized mechanically under ice water. The samples were centrifuged at 3000 rpm for 10 min and the supernatant was collected. The activities of hepatic TC and TG, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total antioxidative capacity (TAOC), are considered indicators of the antioxidant status of liver tissues, were evaluated using commercial kits (Nanjing Jiancheng Biology Engineering Institute, Nanjing, China), and normalized to albumin (BSA) reference levels. Protein concentration was measured by Bradford’s method [20 (link)].
The liver tissues were weighed according to the weight (g): volume (mL) = 1:9 and added to 9 times the volume of homogenate medium accurately. Then, the mixture was homogenized mechanically under ice water. The samples were centrifuged at 3000 rpm for 10 min and the supernatant was collected. The activities of hepatic TC and TG, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total antioxidative capacity (TAOC), are considered indicators of the antioxidant status of liver tissues, were evaluated using commercial kits (Nanjing Jiancheng Biology Engineering Institute, Nanjing, China), and normalized to albumin (BSA) reference levels. Protein concentration was measured by Bradford’s method [20 (link)].
6
Gestational Diabetes Screening Protocol
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Participants were measured for body weight, height, systolic blood pressure (SBP) and diastolic blood pressure (DBP) at the first prenatal visit (6–12 weeks of gestation). SBP and DBP were taken by trained nurses with an automatic blood pressure monitor. Body mass index (BMI) was determined by dividing body weight in kilograms by height in meters squared. Medical history, personal history and family history were asked by attending doctors and recorded in electronic medical records (EMRs).
Blood samples were collected at the first prenatal visit and 75-g OGTT was conducted for all participants during 24–28 weeks of gestation. Biochemical parameters presented in the present study, including ALT, AST, total bilirubin (TBil), total cholesterol (TC), TG, HDL-C and low-density lipoprotein cholesterol (LDL-C), were measured by Beckman AU5800 automatic analyzer. Quality controls were performed by Bio-Rad biochemical quality control product.
GDM was diagnosed according to the International Association of the Diabetes and Pregnancy Study Group's criterion in 2010.1 (link) For all participants in the present study, all available clinical and laboratory data were recorded and verified by two researchers at the same time.
Blood samples were collected at the first prenatal visit and 75-g OGTT was conducted for all participants during 24–28 weeks of gestation. Biochemical parameters presented in the present study, including ALT, AST, total bilirubin (TBil), total cholesterol (TC), TG, HDL-C and low-density lipoprotein cholesterol (LDL-C), were measured by Beckman AU5800 automatic analyzer. Quality controls were performed by Bio-Rad biochemical quality control product.
GDM was diagnosed according to the International Association of the Diabetes and Pregnancy Study Group's criterion in 2010.1 (link) For all participants in the present study, all available clinical and laboratory data were recorded and verified by two researchers at the same time.
7
Immunogenicity Evaluation of HBsAg
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Frozen serum samples collected from the immunized mice were transferred to the reference lab at The First Affiliated Hospital of Wenzhou Medical University for assays of HBsAg and serum ALT levels. The Modular E170 analyzer (Roche Diagnostics, Basel, Switzerland) was employed for HBsAg assay. ALT activity was quantified by the AU5800 automatic analyzer (Beckman Coulter, Inc.).
Statistical analysis. Data analysis was performed using SPSS software (version 18.0; SPSS Inc., Chicago, IL, USA). Data are expressed as the mean ± standard deviation. Statistical analysis was performed using one-way analysis of variance followed by LSD-t or Dunnett's T3 post hoc tests for multi group comparisons. P<0.05 was considered to indicate a statistically significant difference.
Statistical analysis. Data analysis was performed using SPSS software (version 18.0; SPSS Inc., Chicago, IL, USA). Data are expressed as the mean ± standard deviation. Statistical analysis was performed using one-way analysis of variance followed by LSD-t or Dunnett's T3 post hoc tests for multi group comparisons. P<0.05 was considered to indicate a statistically significant difference.
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