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Minipid 200b

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The MiniPID 200B is a compact and sensitive photo-ionization detector (PID) designed for the detection and measurement of volatile organic compounds (VOCs) in air. It features a mini-sized sensor for quick response and portability. The device measures the concentration of VOCs by ionizing the sample and detecting the resulting electrical current.

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MiniPID (18 citations)

7 protocols using minipid 200b

1

Quantifying Volatile Compounds in Sweat

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A PID (200B miniPID, Aurora Scientific, Canada) quantified the number of volatiles given off by the sweat samples. The highly sensitive PID sensor with a 2.25 inch inlet needle drew in air through a suction pump, after which molecules with ionization energy < 10.6 eV were ionized by high-intensity ultraviolet light. This meant that natural constituents of air (e.g. oxygen) were not detected, while the PID did detect sweat molecules (e.g. acetic acid). Ionization caused a current proportional to the molecules’ concentration. The PID sensor head was connected to a miniPID controller (gain: × 5; pump: high). Recordings started when the PID signal was zeroed in LabChart8 after a recommended 30 min warm-up period.
de Groot J.H., Kirk P.A, & Gottfried J.A. (2020). Encoding fear intensity in human sweat. Philosophical Transactions of the Royal Society B: Biological Sciences, 375(1800), 20190271.
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2

Dual PID Discrimination of Organic Compounds

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Two photoionisation detectors (200B miniPID, Aurora Scientific, Aurora ON, Canada) fitted with UV lamps of emission energy 10.6 eV (PID high) and 8.4 eV (PID low) were used to discriminate ethyl butyrate (EB, ionisation energy = 9.9 eV) from α-Terpinene (AT, ionisation energy = 7.6 eV) or ethyl valerate (EV, ionisation energy = 10.0 eV) from tripropyl amine (TA, ionisation energy = 7.2 eV). To accommodate the lower voltage UV lamp, resonance circuitry in the PID headstage electronics was adjusted according to manufacturer’s recommendations. Specifically, potentiometer ‘PT1’ was adjusted up to the point where the 8.4 eV lamp began to glow. Further, we tested if the now converted PID low was now sensitive to only AT and TA while not detecting EB and EV. The PID inlets were connected with a 3-way connector to detect incoming odours by both PIDs simultaneously from a common point. PID heads were held on lab stands with the PID inlet at approximately 4 cm above ground level.
Ackels T., Erskine A., Dasgupta D., Marin A.C., Warner T.P., Tootoonian S., Fukunaga I., Harris J.J, & Schaefer A.T. (2021). Fast odour dynamics are encoded in the olfactory system and guide behaviour. Nature, 593(7860), 558-563.
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3

Validating Odorant Delivery using PID

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A photoionization detector (PID, 200B miniPID, Aurora Scientific®, Canada) was used to validate odor delivery of a tracer odorant. The miniPID was placed in the odor port to validate what the dog was receiving when searching a port during and after a trial. With the miniPID inside the port, we activated the odor valve for 30 s (typical duration of a trial). After the 30 s, the odor line was stopped for 30 s to allow odor clearance and activated again. This cycle was repeated 40 consecutive times. For this test, the olfactometer microcontroller (Arduino) algorithm was modified to run the odor cycle automatically and to send a voltage signal to synchronize valve activation with PID readings. Analog voltage readings from the PID and the microcontroller indicating odor activation were sampled at 30 Hz using a LabJack (U6) DAQ.
The tracer odorant used for this was limonene (CAS:5989-54-8) diluted in mineral oil (10−1v/v) to facilitate odor detection by the sensor due to the PID's poor sensitivity to SP. The odor line was set at 1 LPM and the continuous line was set at 2 LPM. This produced a 33% air dilution, as in training. The continuous airline was on during the odor clearance period.
Aviles-Rosa E.O., Gallegos S.F., Prada-Tiedemann P.A, & Hall N.J. (2021). An Automated Canine Line-Up for Detection Dog Research. Frontiers in Veterinary Science, 8, 775381.
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4

Fluidic Time Response Characterization

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A key parameter of the fluidic system for real-time odor measurements is the time required for filling and cleaning the sensing chamber. To characterize the fluidic time response inside the chamber we used a fast-response photo-ionization detector (PID) with a bandwidth of 300 Hz (miniPID 200B, Aurora Scientific, Canada) to monitor the exhaust of the gas chamber when odor bags of different concentration were connected to the inlet of the e-nose.
Burgués J., Esclapez M.D., Doñate S, & Marco S. (2021). RHINOS: A lightweight portable electronic nose for real-time odor quantification in wastewater treatment plants. iScience, 24(12), 103371.
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5

Olfactory Stimulation and Vascular Dynamics

Cited 2 times
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All experiments were performed with the same stimulation protocol, so no blinding was involved. Odor delivery followed a similar procedure as in previous studies (54 (link), 55 (link)). Odors were delivered with a home-built olfactometer controlled by custom LabVIEW software. Odor and exhaust lines were systematically equilibrated before each experiment to avoid pressure artifacts. Odor concentration and stability over the stimulus were assessed and calibrated using a photoionization detector (miniPID 200B, Aurora Scientific). To check for anesthesia-related variations during the experiment, the different stimulus conditions were randomly interleaved by the experimenter. In Fig. 5 only, linescans were performed only once per glomerulus to detect all responsive glomeruli over the bulb. This was followed by repetitive oxygen measurements in individual capillaries. Odor applications were separated by a 3-min period to allow full neuronal and vascular recovery (56 (link)).
Aydin A.K., Verdier C., Chaigneau E, & Charpak S. (2022). The oxygen initial dip in the brain of anesthetized and awake mice. Proceedings of the National Academy of Sciences of the United States of America, 119(14), e2200205119.
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6

Odor Delivery Calibration in Drosophila

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Odors were presented as previously described [8 ]. In brief, a carrier stream of carbon-filtered house air was continuously presented at 2.2 L/min to the fly. A solenoid was used to redirect 200 ml/min of this air stream into an odor vial before rejoining the carrier stream, thus diluting the odor a further 10-fold prior to reaching the animal. cVA (Pherobank, Wageningen, Netherlands) was delivered as a pure odorant before the 10-fold carrier stream dilution. For all experiments, the odor was presented every 40 s. In Figure 4, we varied the odor strength by adding a solenoid valve between the odor vial and the carrier tube [50 (link)]. This allowed us to flush the odor vial before redirecting the odor to the fly. Flushing the odor vial for longer time periods resulted in lower effective concentrations of the odor at the fly. We verified this olfactometer with a photo-ionization device (Aurora Scientific, Ontario Canada, mini-PID 200B) as seen in Figure S4.
Suzuki Y., Schenk J.E., Tan H, & Gaudry Q. (2020). A population of interneurons signals changes in the basal concentration of serotonin and mediates gain control in the Drosophila antennal lobe. Current biology : CB, 30(6), 1110-1118.e4.
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7

Custom Olfactometer for Odor Delivery

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A home built olfactometer based on the design of the Rinberg lab (https://www.janelia.org/open-science/olfactometer), and controlled by custom Labview software was used to deliver the odors. Pure air was constantly delivered to the mouse nose and a valve switched the flow from air to an odor-air mixture (800 mL min−1) for a 2 s stimulation. The pressure of the air line and the odor line were measured and balanced before starting the experiment. Odor concentrations were calibrated with a photo-ionization detector (miniPID 200B, Aurora Scientific, Aurora, Canada). The air plus odor mixture was supplemented with 200 mL min−1 O2. For awake mice total air flow was 500 mL min−1 of odor-air mixture with no additional O2.
Rungta R.L., Chaigneau E., Osmanski B.F, & Charpak S. (2018). Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia. Neuron, 99(2), 362-375.e4.
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