Proteins were separated by SDS-PAGE on Tris gels (Bio-Rad) and were transferred onto PVDF membranes (Bio-Rad), as previously described (Bomberger et al., 2011 (link)). The following antibodies were used for protein detection: anti-ALIX (EMD Milllipore), anti-calnexin (Santa Cruz Biotechnology), anti-CD81 (Thermo Fisher), anti-Flotillin-1 (BD Biosciences), anti-ferritin (Abcam), anti-GM130 (BD Biosciences), anti-Hsp90 (Enzo Life Sciences), anti-lactoferrin (Santa Cruz Biotechnology), anti-MHC-I (LifeSpan Biosciences), anti-RSV (Meridian Life Science, Inc.), anti-transferrin (Santa Cruz Biotechnology), anti-Tsg101 (GeneTex). Secondary antibodies were goat anti-mouse, goat anti-rabbit, and rat anti-goat conjugated to HRP (Bio-Rad).
Anti mhc 1
Manufactured by LifeSpan BioSciences
The Anti-MHC-I is a monoclonal antibody that specifically binds to the major histocompatibility complex class I (MHC-I) protein. MHC-I is expressed on the surface of most nucleated cells and plays a crucial role in the presentation of foreign or self-peptides to the immune system. The Anti-MHC-I antibody can be used to detect and study the expression and function of MHC-I in various biological and immunological applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti tsg101, by GeneTex (2 mentions)
Anti flotillin 1, by BD (2 mentions)
Anti cd81, by Thermo Fisher Scientific (2 mentions)
Anti hsp90, by Enzo Life Sciences (2 mentions)
Anti ferritin, by Abcam (2 mentions)
Tris gels, by Bio-Rad (2 mentions)
Anti lactoferrin, by Santa Cruz Biotechnology (2 mentions)
Anti calnexin, by Santa Cruz Biotechnology (2 mentions)
Anti transferrin, by Santa Cruz Biotechnology (2 mentions)
Pvdf membrane, by Bio-Rad (2 mentions)
Anti gm130, by BD (2 mentions)
2 protocols using anti mhc 1
1
Protein Separation and Detection via SDS-PAGE and Western Blotting
2
Protein Separation and Detection via SDS-PAGE and Western Blotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Proteins were separated by SDS-PAGE on Tris gels (Bio-Rad) and were transferred onto PVDF membranes (Bio-Rad), as previously described (Bomberger et al., 2011 (link)). The following antibodies were used for protein detection: anti-ALIX (EMD Milllipore), anti-calnexin (Santa Cruz Biotechnology), anti-CD81 (Thermo Fisher), anti-Flotillin-1 (BD Biosciences), anti-ferritin (Abcam), anti-GM130 (BD Biosciences), anti-Hsp90 (Enzo Life Sciences), anti-lactoferrin (Santa Cruz Biotechnology), anti-MHC-I (LifeSpan Biosciences), anti-RSV (Meridian Life Science, Inc.), anti-transferrin (Santa Cruz Biotechnology), anti-Tsg101 (GeneTex). Secondary antibodies were goat anti-mouse, goat anti-rabbit, and rat anti-goat conjugated to HRP (Bio-Rad).
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