An Agilent RRLC 1200 system (Agilent Technologies, Agilent Inc., California, CA, USA) was utilized as the liquid chromatography (LC) delivery system with the use of an Agilent ZORBAX Rapid Resolution HT column (2.1 mm × 100 mm, 1.8 μm of particle size) for separation of AHL molecules and an Agilent 6500 Q-TOF LC/MS system was used for MS analysis. The mobile phases, injection volume, parameters for the MS analysis, ESI-positive mode, precursor ion scan mode targeting at the production ion with m/z 102, m/z value range (m/z 150–400) and Agilent MassHunter software for MS spectra analysis were performed essentially as reported [24 (link)].
6500 q tof lc ms system
Manufactured by Agilent Technologies
Sourced in United States
The 6500 Q-TOF LC/MS system is a high-resolution, accurate-mass liquid chromatography-mass spectrometry (LC-MS) instrument. It is designed to provide precise and reliable analytical data for qualitative and quantitative analysis of complex samples. The system combines a quadrupole time-of-flight (Q-TOF) mass analyzer with a high-performance liquid chromatography (HPLC) system, enabling comprehensive analyte identification and quantification.
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2 protocols using 6500 q tof lc ms system
1
Quantitative AHL Detection by LC-MS
2
Quantitative Profiling of Acyl-Homoserine Lactones
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An Agilent RRLC 1200 system was utilized as the liquid chromatography (LC) delivery system with the use of Agilent ZORBAX Rapid Resolution HT column and the MS parameters were fixed as reported previously [17 (link)]. The high resolution electrospray ionization mass spectrometry (ESI-MS) was performed with an Agilent 6,500 Q-TOF LC/MS system operated in ESI-positive mode. The m/z value range to detect the precursor ions was set at m/z 150–400. The precursor ion scan mode targeting at the production ion with m/z 102. Analysis of MS spectra generated and AHL profile was performed as described previously [17 (link)].
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