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Anti somatostatin

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Anti-somatostatin is a laboratory reagent used in research applications. It is a monoclonal antibody that binds to and neutralizes the somatostatin protein. Somatostatin is a hormone that regulates the release of other hormones in the body. Anti-somatostatin can be used to study the effects of somatostatin in various biological systems.

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Lab products found in correlation

Anti ghrelin, by Santa Cruz Biotechnology (2 mentions) Anti pdx1, by R&D Systems (2 mentions) Anti sox17, by R&D Systems (2 mentions) Anti glucagon, by Merck Group (2 mentions) Foxp3 staining buffer set, by Thermo Fisher Scientific (1 mentions) Anti glucagon, by Cell Signaling Technology (1 mentions) Collagenase, by Merck Group (1 mentions) Vectashield mounting media, by Vector Laboratories (1 mentions) Anti sox9, by Merck Group (1 mentions) Anti insulin, by Agilent Technologies (1 mentions) Anti cleaved caspase 3, by BD (1 mentions) Anti chromogranin a, by Immunostar (1 mentions) Paraformaldehyde solution, by Thermo Fisher Scientific (1 mentions) Anti p smad2 3, by Cell Signaling Technology (1 mentions) Anti oct4, by Santa Cruz Biotechnology (1 mentions) Anti pax6, by Fortrea (1 mentions) Anti cd31, by BD (1 mentions) Anti insulin, by Santa Cruz Biotechnology (1 mentions) Mouse monoclonal anti glucagon, by Merck Group (1 mentions) Dab chromogen system, by Agilent Technologies (1 mentions)

Spelling variants of this product

Somatostatin (9 citations) Rabbit anti-somatostatin (9 citations) Rabbit anti-somatostatin antibody (2 citations) Rabbit anti-human somatostatin (2 citations) Anti-TM (1 citations)

4 protocols using anti somatostatin

1

Differentiation and Characterization of hESC-derived Cells

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hESC-derived cells were dissociated using Accutase. To analyze GFP expression, the cells were resuspended in PBS and used directly for analysis. For intracellular staining, the cells were fixed and stained using Foxp3 staining buffer set (eBiosciences) according to the manufacturer’s instructions. Briefly, cells were first blocked with 2% horse serum for 15 min and then incubated with primary antibody for 45 min at RT, washed twice, incubated with fluorescence-conjugated secondary antibody for 30 min at 4 °C, washed twice and re-suspended in FACS buffer for analysis. The following primary antibodies were used: anti-SOX17 (1:500, R&D), anti-PDX1 (1:500, R&D), anti-pro-insulin (1:500, Millipore), anti-glucagon (1:100, Cell Signaling), anti-somatostatin (1:1000, DAKO), and anti-ghrelin (1:500, Santa Cruz). Samples were analyzed with an Accuri C6 flow cytometry instrument and the data were processed using Flowjo v10 software.
Amin S., Cook B., Zhou T., Ghazizadeh Z., Lis R., Zhang T., Khalaj M., Crespo M., Perera M., Xiang J.Z., Zhu Z., Tomishima M., Liu C., Naji A., Evans T., Huangfu D, & Chen S. (2018). Discovery of a drug candidate for GLIS3-associated diabetes. Nature Communications, 9, 2681.
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2

Whole-Mount Immunohistochemistry of Zebrafish Larvae

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Whole-mount immunohistochemistry was performed as described before [86 (link)], with incubation in 0.1% collagenase (Sigma) in PBS-Tween for 30 min for the digestion step. For immunohistochemistry against glucagon and somatostatin, larvae were deyolked before incubation in blocking buffer. Primary antibodies used were anti-cytokeratin (Santa Cruz Biotechnology, 1:50), anti-glucagon (Sigma, 1:1,000), anti-somatostatin (Dako, 1:200) and anti-carboxypeptidase-a (Sigma, 1:100). The larvae were mounted with Vectashield Mounting Media (Vector Laboratories).
Bielczyk-Maczyńska E., Lam Hung L., Ferreira L., Fleischmann T., Weis F., Fernández-Pevida A., Harvey S.A., Wali N., Warren A.J., Barroso I., Stemple D.L, & Cvejic A. (2015). The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish. PLoS Genetics, 11(12), e1005677.
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3

Immunocytochemistry for Pancreatic Cell Markers

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Cells were fixed in 4% paraformaldehyde solution (Affymetrix) for 20 min, then blocked and permeabilized in PBS solution containing 5% horse serum and 0.3% Triton for 1 h at room temperature. The cells were incubated with primary antibodies overnight at 4 °C followed by 1 h incubation with fluorescence-conjugated secondary antibodies (Alexafluor, ThermoFisher Scientific) at RT. For pSMAD2/3 staining, cells were permeabilized with ice-cold methanol at −20 °C for 10 min after fixation and prior to blocking. The following primary antibodies were used: anti-OCT4 (1:200, Santa Cruz), anti-SOX17 (1:500, R&D), anti-PDX1 (1:500, R&D), anti-SOX9 (1:1000, Millipore), anti-NKX6.1 (1:500, DSHB), anti-NKX2.2 (1:500, DSHB), anti-PAX6 (1:1000, Covance), anti-ISL1 (1:200, DSHB), anti-UCN3 (1:500, Pheonix Pharmaceuticals), anti-NGN3 (1:500, R&D), anti-chromogranin A (1:1000, Immunostar), anti-glucagon (1:2000, Sigma), anti-somatostatin (1:1000, DAKO), anti-ghrelin (1:500, Santa Cruz), anti-insulin (1:500, DAKO), and anti-cleaved caspase-3 (1:1000, BD Biosciences), anti-pSMAD2/3 (1:200, Cell Signaling).
Amin S., Cook B., Zhou T., Ghazizadeh Z., Lis R., Zhang T., Khalaj M., Crespo M., Perera M., Xiang J.Z., Zhu Z., Tomishima M., Liu C., Naji A., Evans T., Huangfu D, & Chen S. (2018). Discovery of a drug candidate for GLIS3-associated diabetes. Nature Communications, 9, 2681.
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4

Immunofluorescence Staining of Pancreas Samples

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All pancreas samples were fixed and cryo-protected in 30% sucrose overnight before freezing, as described before [20 (link), 33 (link), 34 (link)]. Staining with 3,3′-Diaminobenzidine (DAB) was performed using a DAB chromogen system (Dako, Carpinteria, CA, USA). A Biotin-TSA or Cy5-TSA enhancer kit (Fisher Scientific) was used to amplify VEGF signals. GFP was detected by direct fluorescence. Primary antibodies for immunostaining were: guinea pig polyclonal anti-insulin and anti-pancreatic polypeptide (Dako), goat polyclonal anti-VEGF-A (R&D systems, Minneapolis, MN, USA) and anti-insulin (Santa Cruz, CA, USA); rabbit polyclonal anti-somatostatin (Dako) and anti-VEGF-A (Santa Cruz); rat polyclonal anti-CD31 (BD, San Jose, CA, USA) and mouse monoclonal anti-glucagon (Sigma, St Louis, MO, USA). Secondary antibodies were all purchased from Jackson ImmunoResearch. Nuclear staining was performed with Hoechst (BD). When duct cells were labelled with biotin-dolichos biflorus agglutinin (DBA) (Vector Lab, Burlingame, CA, USA), they were detected with Cy5-conjugated streptavidin. Staining, imaging of sections and quantifications were performed as described previously [20 (link), 33 (link), 34 (link)].
Xiao X., Prasadan K., Guo P., El-Gohary Y., Fischbach S., Wiersch J., Gaffar I., Shiota C, & Gittes G.K. (2014). Pancreatic duct cells as a source of VEGF in mice. Diabetologia, 57(5), 991-1000.
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