After an overnight fast of at least 8 h, blood samples were collected from each patient before the surgical procedure. Plasma IGF1 and IGFBP3 were measured by chemiluminescent immunoassay using IMMULITE 2,000 system (Siemens AG, Munich, Germany). The IGF1/IGFBP3 ratio was calculated. Serum insulin and C-peptide were measured by electrochemiluminescence immunoassay on Cobas E601 analyzers (Hoffman-La Roche Ltd., Basel, Switzerland). All biomarkers were examined at the Department of Clinical Laboratory, Ruijin Hospital.
Cobas e 601 analyzers
Manufactured by Roche
Sourced in Switzerland
The Cobas e 601 analyzer is a fully automated immunoassay analyzer designed for clinical laboratories. It performs various immunochemistry tests, including tests for hormones, proteins, and infectious diseases. The Cobas e 601 utilizes electrochemiluminescence technology to provide accurate and reliable results.
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Lab products found in correlation
3 protocols using cobas e 601 analyzers
1
Biomarker Analysis in Surgical Patients
2
Comprehensive Metabolic Panel Analysis
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Fasting blood samples were collected for glucose, insulin, lipids (in selected cases), luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol. Glucose was measured by the hexokinase method (Roche Diagnostics, Cobas Integra, Germany). Insulin levels were measured by immunoradiometric assay (IRMA) (DIAsource ImmunoAssays S.A. Nivelles). Luteinizing hormone levels were measured by DSL-4600 Active LH coated tube (IRMA, Diagnostic Systems Laboratories, Inc., Texas, USA), and the limit of detection was 0.12 IU/L with an intra- and inter-assay (CV) of 4.8%-8.9% and 6.8%-8.9%, respectively. Follicle-stimulating hormone levels were measured by DSL-4700 Active FSH-coated tube (IRMA, Diagnostic Systems Laboratories, Inc.) with an intra- and inter-assay CV of 1.6%-3.6% and 5.6%-7.7%, respectively, and the limit of detection was 0.11 IU/L. Estradiol levels were measured by electrochemiluminescence immunoassay (ECLIA, Cobas e 601 analyzers, Roche Diagnostics) kits with intra- and inter-assay CV of 1.3%-6.1% and 1.9%-7.0%, respectively, and the functional sensitivity was 44 pmol/L.
3
Serum Hormone Measurement Protocols
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Serum P4 and E2 were measured using the commercially available VIDAS® ImmunoDiagnostic Assay System as an automated quantitative enzyme-linked fluorescent assay (bioMérieux, Marcy l’Etoile, France). The assay sensitivity was 0.25 ng/mL for serum P4 and 9 pg/mL for serum E2. The intra-assay coefficient of variations was 3.97–14.30% and 2.2–7.5%, and the inter-assay coefficient of variations was 3.10–24.30% and 3.2–9.5% for serum P4 and E2, respectively. All serum P4 measurements one day prior to warmed blastocyst transfer were performed at 12.00–1.00 pm.
Serum LH was measured using the Cobas e 601 analyzers, employing the Elecsys LH immunoassay (Roche Diagnostics International Ltd, Rotkreuz, Switzerland). The assay uses a sandwich test principle and a measuring range of 0.100–200 IU/L, as defined by the lower detection limit and the maximum of the master curve. The coefficients of variation for repeatability and intermediate precision were 0.6–1.2% and 1.6–2.2%, respectively.
Serum LH was measured using the Cobas e 601 analyzers, employing the Elecsys LH immunoassay (Roche Diagnostics International Ltd, Rotkreuz, Switzerland). The assay uses a sandwich test principle and a measuring range of 0.100–200 IU/L, as defined by the lower detection limit and the maximum of the master curve. The coefficients of variation for repeatability and intermediate precision were 0.6–1.2% and 1.6–2.2%, respectively.
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