For tissue homogenization, all fresh tissue samples were weighed and the tissue lysate was prepared according to the manufactures protocol (Invitrogen, CA, USA). Approximately 50 μg of each tissue was excised and washed with ice-cold PBS.
The level of p53, RB, IL-1, IL-6, IL-11, NF-kB, NF-κ PTPN13, E-cadherin, N-cadherin and TWIST was assessed using Abcam’s p53 Simple Step ELISA® Kit (Abcam, Cambridge, MA, USA), Human Retinoblastoma ELISA® kit (Sigma-Aldrich, Saint Louis, USA), Human Retinoblastoma ELISA® kit (Sigma-Aldrich, Saint Louis, USA), Human IL-6 ELISA® Kit, Human IL-1 beta ELISA® Kit, Human IL-11 ELISA® Kit, NF-kB p65 Transcription Factor Assay® Kit (Abcam, Cambridge, MA, USA), Human Tyrosine-Protein Phosphatase Non-Receptor Type 13 (PTPN13) ELISA Kit (MyBiosource, USA), Human E-Cadherin, N-Cadherin ELISA Kit (Abcam, Cambridge, MA, USA).and TWIST ELISA Kit (Aviva Systems Biology, CA, USA).
The level of p53, RB, IL-1, IL-6, IL-11, NF-kB, NF-κ PTPN13, E-cadherin, N-cadherin and TWIST was assessed using Abcam’s p53 Simple Step ELISA® Kit (Abcam, Cambridge, MA, USA), Human Retinoblastoma ELISA® kit (Sigma-Aldrich, Saint Louis, USA), Human Retinoblastoma ELISA® kit (Sigma-Aldrich, Saint Louis, USA), Human IL-6 ELISA® Kit, Human IL-1 beta ELISA® Kit, Human IL-11 ELISA® Kit, NF-kB p65 Transcription Factor Assay® Kit (Abcam, Cambridge, MA, USA), Human Tyrosine-Protein Phosphatase Non-Receptor Type 13 (PTPN13) ELISA Kit (MyBiosource, USA), Human E-Cadherin, N-Cadherin ELISA Kit (Abcam, Cambridge, MA, USA).and TWIST ELISA Kit (Aviva Systems Biology, CA, USA).