Ab22572

Cells were fixed in 4% paraformaldehyde and were stained with the following primary antibodies: rabbit α-rat Nurr1 (sc-991; 1 : 200; Santa Cruz Biotechnology, Santa Cruz, CA, USA); goat α-human Asc1 (sc-13219; 1 : 200; Santa Cruz Biotechnology); mouse α-human vimentin (V5255; 1 : 200; Sigma, St. Louis, MO, USA); rabbit α-mouse Sox1 (ab22572; 1 : 200; Abcam, Cambridge, UK); mouse α-human Nestin (MAB5326; 1 : 200; Millipore, Billerica, MA, USA); rabbit α-rat Tuj1 (MRB-435p; 1 : 1000; Covance, Princeton, NJ, USA); mouse α-human MAP2 (M2320; 1 : 200; Sigma, St. Louis, MO, USA); rabbit α-rat TH (AB152; 1 : 200; Millipore). Appropriate Alexa Fluor 488 or 594 F (ab′)2 fragment of goat α-rabbit or α-mouse IgG (H+L) antibodies or Alexa Fluor 555 goat α-rabbit IgG (H+L) or Alexa Fluor 594 F (ab′)2 fragment of rabbit α-goat IgG (H+L) (A-11070, A-11020 or A-21428, A-21223; 1 : 2000; Invitrogen) and DAPI (1 μg/mL) counterstain were used for visualization. Fluorescence images were obtained using fluorescence microscopy (Evos, Amgmicro, Life Technologies, Carlsbad, CA, USA) and confocal laser scanning microscopy (Fluoview100, Olympus, Tokyo, Japan).