Ha specific monoclonal antibody

Manufactured by Sino Biological

HA-specific monoclonal antibody is a laboratory reagent used for the detection and quantification of hemagglutinin (HA) proteins. It can be used in various immunoassay techniques, such as ELISA, to identify and measure the presence of HA in samples.

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Lab products found in correlation

Nunc immuno 96 well plates, by Thermo Fisher Scientific (4 mentions) Carbonate bicarbonate buffer, by Thermo Fisher Scientific (4 mentions) Accuskan fc microplate, by Thermo Fisher Scientific (4 mentions) Ha protein, by Sino Biological (4 mentions) Anti mouse igg hrp, by Thermo Fisher Scientific (4 mentions)

4 protocols using ha specific monoclonal antibody

Quantifying Influenza Antibody Levels

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Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well) HA protein (Sino Biological) diluted in carbonate/bicarbonate buffer (Fisher Scientific) overnight at 4°C or 1 hour at 37°C. After washing and blocking with TBS for 1 hour the serum samples were diluted and added to the plate. Serially diluted HA-specific monoclonal antibody (Sino Biological) served as standard. Anti-mouse IgG-HRP (1:20,000; Fisher Scientific) in combination with TMB (Fisher Scientific) solution was used for detection. The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).

Qin Z., Bouteau A., Herbst C, & Igyártó B.Z. (2022). Pre-exposure to mRNA-LNP inhibits adaptive immune responses and alters innate immune fitness in an inheritable fashion. PLoS Pathogens, 18(9), e1010830.

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ELISA for HA-specific Antibodies

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Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well) HA protein (Sino Biological) diluted in carbonate/bicarbonate buffer (Fisher Scientific) overnight at 4 °C or 1 hour at 37 °C. After washing and blocking with TBS for 1 hour the serum samples were diluted and added to the plate. Serially diluted HA-specific monoclonal antibody (Sino Biological) served as standard. Anti-mouse IgG-HRP (1:20,000; Fisher Scientific) in combination with TMB (Fisher Scientific) solution was used for detection. The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).

Ndeupen S., Bouteau A., Herbst C., Qin Z., Jacobsen S., Powers N.E., Hutchins Z., Kurup D., Diba L.Z., Watson M., Ramage H, & Igyártó B.Z. (2022). Langerhans cells and cDC1s play redundant roles in mRNA-LNP induced protective anti-influenza and anti-SARS-CoV-2 immune responses. PLoS Pathogens, 18(1), e1010255.

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Quantitative HA Antibody ELISA

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Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well) HA protein (Sino Biological) diluted in carbonate/bicarbonate buffer (Fisher Scientific) overnight at 4°C or 1 hour at 37 °C. After washing and blocking with TBS for 1 hour the serum samples were diluted and added to the plate. Serially diluted HA-specific monoclonal antibody (Sino Biological) served as standard. Anti-mouse IgG-HRP (1:20,000; Fisher Scientific) in combination with TMB (Fisher Scientific) solution was used for detection. The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).

Qin Z., Bouteau A., Herbst C, & Igyártó B.Z. (2022). Pre-exposure to mRNA-LNP inhibits adaptive immune responses and alters innate immune fitness in an inheritable fashion. bioRxiv.

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ELISA for HA-specific Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well) HA protein (Sino Biological) diluted in carbonate/bicarbonate buffer (Fisher Scientific) overnight at 4 °C or 1 hour at 37 °C. After washing and blocking with TBS for 1 hour the serum samples were diluted and added to the plate. Serially diluted HA-specific monoclonal antibody (Sino Biological) served as standard. Anti-mouse IgG-HRP (1:20,000; Fisher Scientific) in combination with TMB (Fisher Scientific) solution was used for detection. The signals were read at 450 nm using accuSkan FC microplate photometer (Fisher Scientific).

Ndeupen S., Bouteau A., Herbst C., Qin Z., Hutchins Z., Kurup D., Diba L.Z, & Igyártó B.Z. (2021). Langerhans cells and cDC1s play redundant roles in mRNA-LNP induced protective anti-influenza and anti-SARS-CoV-2 responses. bioRxiv.

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