Ab200015 human lactoferrin simplestep elisa kit

LF was determined in serum of 27 patients with acute osteomyelitis by an ELISA assay (ab200015 human lactoferrin simpleStep ELISA Kit Abcam, Cambridge, MA, USA). LF levels were expressed as ng/ml.

The concentrations of Lf in the colostrum samples were measured using quantitative enzyme-linked immunosorbent assays (ab200015—Human Lactoferrin SimpleStep ELISA^® Kit, Abcam, Cambridge, UK). The colostrum samples had been previously defatted by centrifuging at 500× g for 15 min at 4 °C. The aqueous fraction was recentrifuged at 3000× g for 15 min at 4 °C, and the final aqueous fraction was collected. The defatted colostrum samples were diluted 10,000,000 times using the sample diluent reagent provided in the kit. The mean coefficients of variation (CVs) of intra-assay and inter-assay precision were CV% = 5.1% and CV% = 5.4%, respectively. The units used for the Lf concentrations were mg/mL of milk.