Fatty acids were derivatized, identified, and quantified by gas chromatography and flame ionization detection (Thermo Trace 1300 GC, Thermo Fischer Scientific) according to Remias et al. (2020) (link). Carotenoids, chlorophylls, and alpha-tocopherol were extracted with methyl tert-butyl ether and quantified by HPLC with a C30 Carotenoids column (YMC, Willich, Germany) and a diode array detector (Agilent Technologies, Germany) at 450 nm, according to Procházková et al. (2019) (link). The quantitative results were performed in triplicate and the standard derivation (SD) was calculated. Reactor biomass yield (productivity) of a compound class was expressed in dry mass produced per day and liter reactor volume [mg day−1 L−1], neglecting the minor starting biomass of the inoculum. The yields of the stress phase were calculated in the same manner, however by subtracting the starting biomass and respectively the compound dry weights which were already present in the reactors at the start of the stress phase.
Thermo trace 1300 gc
Manufactured by Thermo Fisher Scientific
The Thermo Trace 1300 GC is a gas chromatograph designed for high-performance separation and analysis of a wide range of volatile and semi-volatile compounds. It features advanced hardware and software components to provide reliable and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using thermo trace 1300 gc
1
Quantifying Microalgal Biomass Compounds
2
Quantifying Microalgal Biomass Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fatty acids were derivatized, identified, and quantified by gas chromatography and flame ionization detection (Thermo Trace 1300 GC, Thermo Fischer Scientific) according to Remias et al. (2020) (link). Carotenoids, chlorophylls, and alpha-tocopherol were extracted with methyl tert-butyl ether and quantified by HPLC with a C30 Carotenoids column (YMC, Willich, Germany) and a diode array detector (Agilent Technologies, Germany) at 450 nm, according to Procházková et al. (2019) (link). The quantitative results were performed in triplicate and the standard derivation (SD) was calculated. Reactor biomass yield (productivity) of a compound class was expressed in dry mass produced per day and liter reactor volume [mg day−1 L−1], neglecting the minor starting biomass of the inoculum. The yields of the stress phase were calculated in the same manner, however by subtracting the starting biomass and respectively the compound dry weights which were already present in the reactors at the start of the stress phase.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!