Glibenclamide (GLB) and Streptozotocin (STZ) were obtained from Sigma Aldrich chemicals (St. Louis, MO, USA). Radioimmunoassay kit for the rat insulin was obtained from Amersham Biosciences; (Piscataway, NJ, USA). The glucose oxidase kit was purchased from Randox, UK. The Glucotest (glucose urine strips) was obtained from Roche Diagnostics Germany (Mannheim, Germany). Thiobarbituric acid for measurement of lipid peroxidation (LPO) was purchased from Fluka (Buchs, Switzerland). All remaining chemicals were of the highest grade and commercially available.
Glibenclamide glb
Manufactured by Merck Group
Sourced in United States
Glibenclamide (GLB) is a pharmaceutical compound used as a laboratory reagent. It is a sulfonylurea derivative that functions as an anti-diabetic drug by stimulating insulin secretion from pancreatic beta cells.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (1 mentions)
Kt5823, by Merck Group (1 mentions)
Nicardipine, by Merck Group (1 mentions)
Bay k 8644, by Cayman Chemical (1 mentions)
N gamma nitrol arginine methyl ester l name, by Merck Group (1 mentions)
Isopentenyl pyrophosphate ipp, by Merck Group (1 mentions)
Tetraethyl ammonium tea, by Merck Group (1 mentions)
Serotonin 5 ht, by Merck Group (1 mentions)
4 aminopyridine 4 ap, by Merck Group (1 mentions)
Whatman, by Cytiva (1 mentions)
Stat3 inhibitor 6, by Santa Cruz Biotechnology (1 mentions)
9 phenanthrol 9 phe, by Merck Group (1 mentions)
Kg 501, by MedChemExpress (1 mentions)
Lipopolysaccharide lps, by Solarbio (1 mentions)
5 protocols using glibenclamide glb
1
Streptozotocin-Induced Diabetic Rat Model
2
Pharmacological Modulation of Signaling Pathways
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The following drugs were obtained from the sources: Glibenclamide (GLB) and KT5823 were obtained from Sigma Aldrich (St. Louis, MO, United States). 1H-[1,2,4]oxadiazolo[4,3,-a]quinoxalin-1-one (ODQ) was purchased from Calbiochem (San Diego, CA, United States). Ruthenium NO donor [Ru(bpy)2(NO)SO3](PF6) was synthesized and used as previously described (Silva et al., 2006 (link)). ODQ and KT5823 were diluted in dimethylsulfoxide (DMSO) 2% in saline, and GLB was diluted in Tween 80 5% in saline.
3
Preparation of Krebs-Ringer Bicarbonate Solutions
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Potassium chloride (KCl), sodium chloride (NaCl), magnesium sulphate (MgSO 4 ), calcium chloride (CaCl 2 ), glucose (C 6 H 12 O 6 ), potassium phosphate (KH 2 PO 4 ), sodium carbonate (NaHCO 3 ), barium chloride (BaCl 2 ), and ethylenediaminetetraacetic acid (EDTA) were obtained from Lach-Ner s.r.o. (Neratovice, Czech Republic) and used to prepare Krebs-Ringer bicarbonate solution and Ca 2+ -free Krebs-Ringer bicarbonate solution.
Other substances: serotonin (5-HT; purity ≥98%), nicardipine (NCP; purity ≥98%), CRV (purity ≥98%), N (gamma)-nitrol-arginine methyl ester (L-NAME; purity ≥98%), tetraethylammonium (TEA; purity ≥99.5%), 4-aminopyridine (4-AP; pu-rity ≥99%), glibenclamide (GLB; purity ≥ 99%), and isopentenyl pyrophosphate (IPP; purity ≥95%) were purchased from Sigma Aldrich (St. Louis, MO, USA), while (±) BAY K 8644 (purity ≥97.5%) was purchased from Cayman Chemical Company. Their stock solutions were prepared either in distilled water or in 99.9% ethanol, according to their solubility, and the subsequent dilutions were carried out in distilled water. All solutions were kept at 0-4 • C when not used in experiments.
Other substances: serotonin (5-HT; purity ≥98%), nicardipine (NCP; purity ≥98%), CRV (purity ≥98%), N (gamma)-nitrol-arginine methyl ester (L-NAME; purity ≥98%), tetraethylammonium (TEA; purity ≥99.5%), 4-aminopyridine (4-AP; pu-rity ≥99%), glibenclamide (GLB; purity ≥ 99%), and isopentenyl pyrophosphate (IPP; purity ≥95%) were purchased from Sigma Aldrich (St. Louis, MO, USA), while (±) BAY K 8644 (purity ≥97.5%) was purchased from Cayman Chemical Company. Their stock solutions were prepared either in distilled water or in 99.9% ethanol, according to their solubility, and the subsequent dilutions were carried out in distilled water. All solutions were kept at 0-4 • C when not used in experiments.
4
Extraction and Characterization of Codonopsis lanceolata
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An extract of Codonopsis lanceolata (ECL) was prepared as previously described [19 (link)]. Briefly, sliced CL roots obtained from Panax Korea were lyophilized and extracted at 60 °C for 270 min, using 55% ethanol using a condenser. The extract was filtered (Whatman Inc., Clifton, NJ, USA) to remove undissolved residue, concentrated using a rotary evaporator (N-1000S; EYELA, Tokyo, Japan), and lyophilized into a powder. Phenylephrine (PE), acetylcholine (ACh), Nω-nitro-L-arginine methyl ester (L-NAME), potassium chloride (KCl), calcium chloride (CaCl2), ethylene glycol-bis (β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), tetraethylammonium (TEA), and glibenclamide (GLB) were purchased from Sigma Aldrich (St. Louis, MO, USA). All chemicals were dissolved in distilled water with the exception of GLB, which was dissolved in dimethyl sulfoxide.
5
Pharmacological Modulators of Signal Pathways
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Reagents Lipopolysaccharide (LPS) was purchased from Solarbio (Peking, China). Glibenclamide (GLB, an inhibitor of SUR1) and 9-phenanthrol (9-Phe, an inhibitor of TRMP4) were purchased from Sigma-Aldrich (St.Louis, MO, USA). TGFα recombinant proteins were purchased from Abcam (Cambridge, UK). CaMKII-IM-1 (an inhibitor of CaMKII) and KG-501 (an inhibitor of CREB) were purchased from MedChemExpress (NJ, USA). NCGC00067819 (an agonist of CREB) was purchased from Probechem (IL, USA). STAT3 inhibitor VI was purchased from Santa Cruz (CA, USA).
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