Lyra 3 microscope

Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, St. Louis, MA, USA) (with MOPS, pH 7.2), dimethylsulfoxide (DMSO), Sabouraud dextrose agar (SDA; Difco), saline solution (SS), iMarKTM microplate reader (Bio-Rad, Hercules, CA, USA), FLC, Bioscreen C equipment, Bioscreen software (Growth Curves USA, Piscataway, NJ, USA), 100-well microplates (honeycomb), LIVE/DEAD™ Yeast Viability kit (Thermo Fisher Scientific, Waltham, MA, USA), Olympus FV 1000 confocal microscope, Tescan Lyra 3 microscope, Fmoc-Arg(Pbf)–OH, Fmoc-Trp(Boc)–OH, Fmoc-Gln(Trt)–OH, Fmoc-Leu-OH, Fmoc-Lys(Fmoc)–OH, Fmoc-6-Ahx-OH, Rink amide resin, dicyclohexilcarbodiimide (DCC), and 1-hydroxy-6-chlorobenzotriazole were purchased from AAPPTec (Louisville, KY, USA). Trifluoroacetic acid (TFA), acetonitrile (ACN), dichloromethane (DCM), N,N-dimethylformamide, ethanodithiol, triisopropylsilane, methanol, acetonitrile, and isopropanol were obtained from Merck (Darmstadt, Germany). SPE SupelcleanTM columns were purchased from Sigma-Aldrich (St. Louis, MO, USA).

The fiber structure was observed by scanning electron microscopy on a LYRA3 microscope (Tescan, Brno, the Czech Republic) and a Helios NanoLab 660 (Thermo Fisher Scientific, Waltham, MA, USA). Due to the polymeric nature of the sample, the fibers of the material begin to charge and repel with each other as the charge accumulates, resulting in the movement of the fibers making it difficult to focus and scan at high magnification. Therefore, the samples for analysis were carbonized on a Coater EM ACE600 instrument (Leica Microsystems, Wetzlar, Germany) for analysis. Several images were selected for each sample, and the mean of ten randomly selected fibers was measured. Newly occurring defects were also observed. The following parameters were chosen for all SEM observations: detector SE, acceleration voltage 5kV, working distance 9 mm (LYRA3) and 4 mm (Helios), magnification from 5 k× to 80 k×.