CSF samples were collected using polypropylene tubes at the end of lumbar punctures and centrifuged within 2 hours at 1,500 ✕ g for 10 minutes according to the guidelines of the BioMS-eu consortium, except that sample transport and centrifugation were performed at 4°C instead of room temperature.27 (link) Aliquots (500 µL) were stored at −80°C in 1.5 mL tubes (Protein LoBind 0030108.116, Eppendorf) until use. Patients with traumatic lumbar punctures (>500 red cells/mm3) were excluded.
Serum samples were spun at the latest 2 hours after blood sampling at 1,500 × g for 10 minutes at room temperature and aliquots (500 µL) stored at −80°C in 1.5 mL tubes (Protein LoBind 0030108.116, Eppendorf) until use.
Rival M., Thouvenot E., Du Trieu de Terdonck L., Laurent-Chabalier S., Demattei C., Uygunoglu U., Castelnovo G., Cohen M., Okuda D.T., Kantarci O.H., Pelletier D., Azevedo C., Marin P., Lehmann S., Siva A., Mura T, & Lebrun-Frenay C. (2022). Neurofilament Light Chain Levels Are Predictive of Clinical Conversion in Radiologically Isolated Syndrome. Neurology® Neuroimmunology & Neuroinflammation, 10(1), e200044.
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