Glucose was measured by a YSI 2300 or 2700 autoanalyser (Yellow Springs Instruments, Yellow Springs, OH, USA). NEFA was measured using a colorimetric method (Wako Pure Chemical Industries, Richmond, VA, USA). Insulin was measured with an ELISA originally developed for human serum or plasma (Linco Research, St Charles, MO, USA) and adapted for dog plasma. The method is based on two murine monoclonal antibodies that bind to different epitopes of insulin but do not bind proinsulin [13 (link)]. Cortisol and catecholamines were measured using commercially available kits (cortisol RIA kit from Diagnostic Products, Los Angeles, CA, USA; catecholamines ELISA from Rocky Mountain Diagnostics, Colorado Springs, CO, USA).
Colorimetric method
Manufactured by Fujifilm
Sourced in United States, Japan
The Colorimetric method is a laboratory equipment used for the analysis and measurement of various chemical compounds and materials. It operates by determining the concentration of a substance through the analysis of the color intensity of a chemical reaction. This method is widely used in various industries and research fields for the accurate quantification of different analytes.
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Lab products found in correlation
2 protocols using colorimetric method
1
Measuring Biomarkers in Dog Plasma
2
Metabolic Markers in Clinical Trial
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A YSI 2300 STAT Plus glucose analyzer was used to analyze PG.
Insulin, C-peptide, glucagon, and free FA (FFA) concentrations were measured at t = 0, 210, 270, 390, and 450 min. Insulin (no. 10-1113-01), glucagon (no. 10-1271-01) and C-peptide concentrations (no. 10-1136-01) were measured by ELISA (Mercodia, Uppsala, Sweden). Serum FFA concentrations were measured by a colorimetric method (Wako Pure Chemical Industries, Osaka, Japan). VLDL-TG and total TG concentrations were analyzed on a cobas 111 (F. Hoffmann–La Roche), using a glycerol blanked kit.
Insulin, C-peptide, glucagon, and free FA (FFA) concentrations were measured at t = 0, 210, 270, 390, and 450 min. Insulin (no. 10-1113-01), glucagon (no. 10-1271-01) and C-peptide concentrations (no. 10-1136-01) were measured by ELISA (Mercodia, Uppsala, Sweden). Serum FFA concentrations were measured by a colorimetric method (Wako Pure Chemical Industries, Osaka, Japan). VLDL-TG and total TG concentrations were analyzed on a cobas 111 (F. Hoffmann–La Roche), using a glycerol blanked kit.
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