Two-year-old P. ginseng Meyer was obtained from the Ginseng Resource Bank, Kyung Hee University. To grow ginseng plants, artificial soil was prepared by mixing vermiculite, perlite, and peat moss at a 3:1:1 volume ratio. The mixed soil was autoclaved at 121 °C for 1 h and then air-dried. The sterilization step was repeated twice on different days. Before culturing the roots, tap water was sterilized to prepare the soil mixture at a 25% v/v ratio that was used to fill trays or pots for ginseng growth. Each tray or pot was placed in a 60 cm × 100 cm (0.6 m2) open cabinet inside a closed controlled chamber. We adjusted the photoperiod to a light:dark (16:8h) cycle using lamps (Philips TLD-RS-FLR32SSEX-D 865K) equal to 9500 lux for each covered area. The temperature was controlled at 25 ± 2 °C, and the moisture level was maintained at 60 ± 5%. Sterilized tap water was sprayed onto the soil surface daily, and watering was conducted once a week using sterilized tap water from a plate beneath the tray/pot.
Tld rs flr32ssex d 865k
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The TLD-RS-FLR32SSEX-D 865K is a laboratory equipment product from Philips. It is designed to provide consistent and reliable lighting for various laboratory applications. The product specifications and core function are limited to the information available, and no further interpretation or extrapolation is provided.
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3 protocols using tld rs flr32ssex d 865k
1
Cultivation of 2-year-old Panax ginseng
2
Cultivation of Ginseng Root Seedlings
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We obtained 2- and 4-year-old P. ginseng root seedlings from the Ginseng Resource Bank, Kyung Hee University. As the growth medium, we prepared artificial soil by mixing vermiculite, perlite, and peat moss at a 3:1:1 volume ratio. The mixed soil was autoclaved at 121°C for 1 h and then air-dried. The sterilization step was repeated twice on different days. Before culturing the roots, we added sterilized tap water to the mixed soil in a 25% v/v ratio. Later, we used the prepared soil to fill trays or pots for growing the ginseng. Each tray or pot was placed in an open cabinet with 60 × 100 cm dimension (0.6 m2) inside a closed room. We adjusted the photoperiod to a 16:8-h light:dark cycle using lamps (Philips TLD-RS-FLR32SSEX-D 865K) equal to 9500 lux for each covered area. The temperature was controlled at 25 ± 2°C, and the moisture level was maintained at 60 ± 5%. Sterilized tap water was sprayed onto the soil surface daily, and watering was done using sterilized tap water once a week from a plate beneath the tray/pot.
3
Evaluating Plant Growth Promotion by Bacteria
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Based on the correlation between OD600 and CFU/mL determined in Section 2.4 , 104–1012 CFU/mL of DCY119T suspensions were prepared. Two-year-old seedlings were first dipped into the different bacterial suspensions for 10 min and then planted in pots (11 cm high and 11 cm diameter) filled with a sterilized soil mixture (vermiculite, perlite, and peat moss at a 3:1:1 ratio to which 25% SDW (v/v) had been added). Seedlings dipped into water without bacteria were the negative control. Each treatment group was replicated in three pots, and each pot contained five seedlings. The temperature was controlled at 25±2oC, and moisture levels were maintained at 60 ± 5%. The photoperiod was adjusted to 16 h of day-time and 8 h of night-time with lamps (Philips TLD-RS-FLR32SSEX-D 865K) that provided 9500 lux for each covered area within the chambers. Seedlings were watered once weekly with sterilized tap water, which was applied to the bottom of the pots. The symptom development was observed 10 days after inoculation (when leaves of seedlings were opened), sampling and morphological observations were conducted. The biomass (fresh weight [FW] and dry weight [DW]) of each group was also measured for compatibility analysis.
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