Cidec

Manufactured by Abcam

Sourced in United States

Cidec is a protein that plays a role in lipid droplet formation and adipocyte differentiation. It is commonly used in research related to lipid metabolism and obesity.

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Lab products found in correlation

2 protocols using cidec

Antibody Profiling for Multimodal Analysis

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The following antibodies were used in this study for either western blotting, ChIP-sequencing, immunohistochemistry and chromatin immunoprecipitation. SOD2 (The Binding site, PC096), Gpx1 and Gpx4 (gift from L. Chavatte), ChREBP (Novus, NB400), SCD1 (Cell Signaling, C12H5), Phf2 (Cell Signaling, D45A2), H3K9me1 (Abcam, ab8896), H3K9me2 (Abcam, ab1220), H3K9me3 (Abcam, ab8898), histone H3 (Diagenode, C15200011), p(S473)-AKT (Cell signaling, D9E), Akt (Cell Signaling, 9272), P-p70S6K (Cell Signaling, 108D2), p70S6K (Cell Signaling, 49D7), p-GSK3β (Cell Signaling, D17D2), GSK3β (Cell Signaling, 27C10), LPK (Abcam, ab6191), ACC (Cell Signaling, 3662), FAS (Cell signaling, C20G5), p62 (gift from A. F. Burnol), Nrf2 (Santa Cruz Biotechnology, 13032), G6PDH (Cell Signaling, 12263), TKT (Cell Signaling, 8616), α-SMA (Novus, NB-600-531), collagen I (Novus, NB600-408), GAPDH (Santa Cruz Biotechnology, FL-335), malic enzyme (Novus, 68578), MTTP (Novus, 62489), NQO1 (Novus, A180), RNA PolII (Santa Cruz, sc899), HSP90 (Cell Signaling, 4874), Cidec (Abcam, ab77115), Plin2 (Progen, ap125), G0S2 (Santa Cruz Biotechnology, N13), and FLAG (Sigma, F1804).

Bricambert J., Alves-Guerra M.C., Esteves P., Prip-Buus C., Bertrand-Michel J., Guillou H., Chang C.J., Vander Wal M.N., Canonne-Hergaux F., Mathurin P., Raverdy V., Pattou F., Girard J., Postic C, & Dentin R. (2018). The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity. Nature Communications, 9, 2092.

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Immunohistochemical Analysis of Apoptosis Markers

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The paraffin enwrapped tissue sections were conventionally dewaxed, heated with microwave for 15 min, incubated with the first antibody of 1:200 diluted CIDEC (Abcam, Cambridge, MA, USA), 1:1000 diluted cleaved caspase 3 (C‐caspase3; Cell Signaling Technology, Danvers, MA, USA), 1: 30 diluted cleaved PARP (C‐PARP; Boster Biological Technology, Wuhan, China), TGF‐β1 (Proteintech, Rosemont, IL, USA), collagen 1 and collagen 4 (Abcam) at 4℃ overnight, respectively, then incubated with the second antibody for 1h at 37℃. The results were observed and photographed under FV1000 laser scanning confocal microscope (Olympus, Tokyo, Japan). The IOD values were measured and analyzed by Image‐Pro Plus 5.0 automatic image analysis system (Media Cybernatics, Houston, TX, USA).

Zheng G., Tan Y., Shang Y., Liu Y., Hu B., Wang D., Han L., Wang Z., Zhang W., Ti Y, & Zhong M. (2021). CIDEC silencing attenuates diabetic nephropathy via inhibiting apoptosis and promoting autophagy. Journal of Diabetes Investigation, 12(8), 1336-1345.

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