The BT474, the Skbr-3 and the SUM225 cells were purchased from ATCC, and the JIMT-1 cells were purchased from DSMZ, Germany. All cells were grown as recommended by the respective companies. Cells with as few as 10 culture passages were used for most of the experiments. Mycoplasma contamination was monitored by PCR analysis from time to time using commercially available kits. In addition, normoxin (NC9273499, Fisher Scientific) that has an anti-mycoplasma effect is used in the cell growth media. The anti-HER2 antibodies used were a monoclonal antibody (610162) from BD Biosciences, and a rabbit anti-pTyr1221/1222-HER2 (2243) and a rabbit anti-HER2 (2165) from Cell Signaling Technologies. While the BD antibody recognizes the extracellular region (amino acids 182–373), the Cell Signaling antibodies recognize the cytoplasmic autophosphorylation site. The other antibodies used were anti-SHP2 (610822) from BD Biosciences, rabbit anti-pan-ERK2 (SC-81457) from Santa Cruz Biotechnology, anti-β-actin (A5441) from Sigma-Aldrich, anti-phospho-ERK1/2 (9101S), anti-phospho-Akt (9271S), and anti-pan-Akt (11E7) from Cell Signaling. All the antibodies are extensively characterized as evidenced by a large body of literature, including our own (17 (link), 20 (link)–23 (link), 37 (link)).
Anti shp2 610822
Manufactured by BD
Sourced in United States
Anti-SHP2 (610822) is a laboratory reagent used for research purposes. It is an antibody that specifically targets the SHP2 protein, which is a protein tyrosine phosphatase involved in various cellular signaling pathways. This product is intended for use in research applications such as Western blotting, immunoprecipitation, and other experimental techniques to study the role and function of the SHP2 protein.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin a5441, by Merck Group (3 mentions)
Anti pan akt 11e7, by Cell Signaling Technology (3 mentions)
Normoxin, by Thermo Fisher Scientific (2 mentions)
Bt474, by American Type Culture Collection (2 mentions)
Sum225, by American Type Culture Collection (2 mentions)
Rabbit anti ptyr1221 1222 her2 2243, by Cell Signaling Technology (2 mentions)
Rabbit anti her2 2165, by Cell Signaling Technology (2 mentions)
Rabbit anti pan erk2 sc 81457, by Santa Cruz Biotechnology (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Jimt1 cells, by Leibniz Institute DSMZ (2 mentions)
Anti ubiquitin sc 271289, by Santa Cruz Biotechnology (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Mda mb 468, by American Type Culture Collection (1 mentions)
3 protocols using anti shp2 610822
1
Characterization of HER2-positive Breast Cancer Cell Lines
2
Characterization of HER2-positive Breast Cancer Cell Lines
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The BT474, the Skbr-3 and the SUM225 cells were purchased from ATCC, and the JIMT-1 cells were purchased from DSMZ, Germany. All cells were grown as recommended by the respective companies. Cells with as few as 10 culture passages were used for most of the experiments. Mycoplasma contamination was monitored by PCR analysis from time to time using commercially available kits. In addition, normoxin (NC9273499, Fisher Scientific) that has an anti-mycoplasma effect is used in the cell growth media. The anti-HER2 antibodies used were a monoclonal antibody (610162) from BD Biosciences, and a rabbit anti-pTyr1221/1222-HER2 (2243) and a rabbit anti-HER2 (2165) from Cell Signaling Technologies. While the BD antibody recognizes the extracellular region (amino acids 182–373), the Cell Signaling antibodies recognize the cytoplasmic autophosphorylation site. The other antibodies used were anti-SHP2 (610822) from BD Biosciences, rabbit anti-pan-ERK2 (SC-81457) from Santa Cruz Biotechnology, anti-β-actin (A5441) from Sigma-Aldrich, anti-phospho-ERK1/2 (9101S), anti-phospho-Akt (9271S), and anti-pan-Akt (11E7) from Cell Signaling. All the antibodies are extensively characterized as evidenced by a large body of literature, including our own (17 (link), 20 (link)–23 (link), 37 (link)).
3
Breast Cancer and Fibroblast Cell Lines
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MDA-MB-231, MDA-MB-468, and MCF-10A cells were purchased from the American Type Culture Collection (Manassas, VA, USA), and the mouse embryonic fibroblasts (MEFs) were received from Dr. Steven Frisch (West Virginia University). With regard to human mammary luminal epithelial (HMLE) cells, only cell lysates received from Dr. Alexey Ivanov (West Virginia University) were used. The conditions for cell growth were described previously [21 (link), 25 (link)]. Antibodies used in the study were anti-EGFR (610017) and anti-SHP2 (610822) from BD Biosciences (San Jose, CA, USA); anti-CBL (sc-1651), anti-pan-extracellular signal-regulated kinase 2 (anti-pan-ERK2; sc81457), and anti-ubiquitin (sc271289) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-β-actin (A5441) from Sigma-Aldrich (St. Louis, MO, USA); and anti-phospho-ERK1/2 (9101S), anti-phospho-Akt (9271S), and anti-pan-Akt (11E7) from Cell Signaling Technology (Danvers, MA, USA).
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