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Anti cd154 pe

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Anti-CD154-PE is a fluorescently-labeled antibody that binds to the CD154 (also known as CD40 Ligand) protein expressed on the surface of activated T cells. It is used in flow cytometry applications to detect and quantify CD154-positive cells.

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Lab products found in correlation

Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Anti cd90 fitc, by BD (1 mentions) Anti cd80 fitc, by BD (1 mentions) Anti cd40 pe, by BD (1 mentions) Anti cd34 pe, by BD (1 mentions) Anti cd105 fitc, by BD (1 mentions) Anti cd45 pe, by BD (1 mentions) Anti hla abc fitc, by BD (1 mentions) Anti hla dr fitc, by BD (1 mentions) Anti cd86 pe, by BD (1 mentions) Anti cd73 pe, by BD (1 mentions) Facscalibur, by BD (1 mentions) Live dead fixable violet stain, by Thermo Fisher Scientific (1 mentions) Facsaria 2 instrument, by BD (1 mentions) Aim 5 medium, by Thermo Fisher Scientific (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions)

Close spelling variants of this product

PE mouse Anti-Human CD154 PE-conjugated anti-CD154 (TRAP1, PE-CF594-anti-CD154 CD154-PE Anti-CD154 CD154

3 protocols using anti cd154 pe

1

Confirming 3T3 IL-2/IL-21/CD40L Cell Line

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Initial characterization of the 3T3 IL-2/IL-21/CD40L cell line was performed to confirm continued CD40L expression. CD40L expression on non-irradiated 3T3 IL-2/IL-21/CD40L cells was confirmed by flow cytometry after 1 week (3 passages) and 8 weeks (24 passages). Cells were detached from the flask using cold PBS supplemented with 5 mM EDTA (Gibco) and a portion of the sample was transferred to FACS tubes. Cells were incubated with anti-CD154 PE (BD Biosciences, TRAP-1) in stain media (1× PBS, 10% FBS) for 20 min at 4 °C. The sample was washed with PBS and run on an LSR II (BD Biosciences) alongside an unstained cell sample. Data were analyzed using FlowJo v9.4 (BD Biosciences).
Whaley R.E., Ameny S., Arkatkar T., Seese A., Wall A., Khan I., Carter J.J., Scherer E.M., Rawlings D.J., Galloway D.A., McElrath M.J., Cohen K.W, & McGuire A.T. (2020). Generation of a cost-effective cell line for support of high-throughput isolation of primary human B cells and monoclonal neutralizing antibodies. Journal of Immunological Methods, 488, 112901.
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2

Immunophenotypic Characterization of MSCs

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Aliquots of 1 × 105 MSCs were washed and suspended in PBS supplemented with 0.1% FBS. Cells were incubated with anti-CD34-PE, anti-CD45-PE, anti-CD73-PE, anti-CD90-FITC, anti-CD105-FITC, anti-CD80-FITC, anti-CD86-PE, anti-CD154-PE, anti-CD40-PE, anti-HLA-ABC-FITC, or anti-HLA-DR-FITC antibodies (all from BD, San Diego, CA, USA) for 30 minutes at 4°C and then washed twice in PBS containing 0.1% FBS. Cells were then resuspended in 200 μL PBS containing 0.1% FBS and analyzed at 10,000 events per test using the FACSCalibur (BD Biosciences) or NovoCyte (ACEA Biosciences Inc., San Diego, CA, USA). Data were analyzed using NovoExpress 1.2.1 software.
Kim J.H., Jo C.H., Kim H.R, & Hwang Y.I. (2018). Comparison of Immunological Characteristics of Mesenchymal Stem Cells from the Periodontal Ligament, Umbilical Cord, and Adipose Tissue. Stem Cells International, 2018, 8429042.
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3

Isolation and analysis of activated CD4+ T cells

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PBMCs were isolated from patient blood samples by density gradient centrifugation (Ficoll-Paque Plus; GE Healthcare). Fresh PBMC were cultured in AIM V medium (Gibco) for 20 h with 100 μg/ml peanut protein extract. The peanut extract was prepared by agitating defatted peanut flour (Golden Peanut and Tree Nuts) with PBS, centrifugation, and sterile-filtering. anti-CD154-PE (clone TRAP1; BD Biosciences) was added to the cultures for the last 3 h. After harvesting, the cells were labeled with anti-CD3-AF700 (clone UCHT1), anti-CD4-APC-Cy7 (RPA-T4), anti-CD45RA-FITC (HI100), anti-CD154-PE (all from BD Biosciences), anti-CD69-AF647 (FN50; BioLegend), and Live/Dead Fixable Violet stain (L34955; Thermo Fisher). Live CD3+CD4+CD45RA− activated CD154+ were sorted with a FACSAria II instrument (BD Biosciences). The sorted cells were processed via Seq-Well18 (link).
Tu A.A., Gierahn T.M., Monian B., Morgan D.M., Mehta N.K., Ruiter B., Shreffler W.G., Shalek A.K, & Love J.C. (2019). TCR sequencing paired with massively-parallel 3’ RNA-seq reveals clonotypic T cell signatures. Nature immunology, 20(12), 1692-1699.
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