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Duoset lipocalin elisa kit

Manufactured by R&D Systems
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The DuoSet Lipocalin ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of Lipocalin levels in cell culture supernates, serum, plasma, and urine samples.

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DuoSet ELISA kits (1131 citations) DuoSet ELISA (635 citations) ELISAs (144 citations) DuoSets (49 citations) Mouse Lipocalin-2/NGAL DuoSet ELISA kit (18 citations) Lipocalin-2/NGAL DuoSet ELISA kit (5 citations) Mouse lipocalin-2 DuoSet ELISA kit (3 citations) Lipocalin-2/NGAL DuoSet ELISA Development kit (3 citations) Duoset murine lipocalin-2 ELISA kit (3 citations)

2 protocols using duoset lipocalin elisa kit

1

Lipocalin 2 Detection in Fecal Samples

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Lipocalin 2 was detected in feces homogenized in 500 μl sterile PBS by ELISA (DuoSet Lipocalin ELISA kit (DY1857, R&D Systems, Minneapolis, MN, USA).
Nguyen B.D., Miguelangel C.V., Hartl J., Gül E., Bauer R., Meile S., Rüthi J., Margot C., Heeb L., Besser F., Escriva P.P., Fetz C., Furter M., Laganenka L., Keller P., Fuchs L., Christen M., Porwollik S., McClelland M., Vorholt J.A., Sauer U., Sunagawa S., Christen B, & Hardt W.D. (2020). Import of aspartate and malate by DcuABC drives H2/fumarate respiration to promote initial Salmonella gut-lumen colonization in mice. Cell host & microbe, 27(6), 922-936.e6.
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2

Quantifying Lipocalin 2 and Antibody Isotypes

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Lipocalin 2 was detected in feces homogenized in 500 μl sterile PBS by ELISA using the DuoSet Lipocalin ELISA kit (DY1857, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. Total concentrations of antibody isotypes in mouse serum or intestinal lavages were determined by sandwich ELISA. Coating antibodies were goat anti-mouse IgA (1040-01, SouthernBiotec, Birmingham, AL, USA), goat-anti-mouse IgG2b (1090-01, SouthernBiotec), goat-anti-mouse IgG1 (1070-01, SouthernBiotec), and goat-anti-mouse IgM (1020-01, SouthernBiotec). Detection antibodies were HRP-conjugated anti-mouse IgA α chain (A4789, Sigma-Aldrich), anti-mouse IgG γ chain (A3673, Sigma-Aldrich), and anti-mouse IgM μ chain (A8786, Sigma-Aldrich). Standards were purified mouse IgA (03101D, Pharmingen) or mouse reference serum (RS10-101, Bethyl, Montgomery, TX, USA).
Moor K., Wotzka S.Y., Toska A., Diard M., Hapfelmeier S, & Slack E. (2016). Peracetic Acid Treatment Generates Potent Inactivated Oral Vaccines from a Broad Range of Culturable Bacterial Species. Frontiers in Immunology, 7, 34.
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