The aforementioned gel particles were subjected to peptide mass fingerprinting (PMF) analysis by MALDI-TOF/TOF (ABI 4800 Proteomic Analyzer; Applied Biosystems; Thermo Fisher Scientific, Inc.). PMF was assimilated when protein content was within a range of 800–4,000 Da. Subsequently, 10 most intense peaks were selected to obtain tandem mass spectrometry (MS/MS) data. Conjunction search was conducted with MS and MS/MS. Results with a total score >64 and a match of >4 peptide fragments (best ion score >30; P<0.05) were accepted. The bioinformatics data of PMF by MS and MS/MS were searched by Mascot engine (Version 2.1, Matrix Science, Ltd., London, UK) in MSDB and Swiss-Prot database (25 (link)).
Abi 4800 proteomic analyzer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ABI 4800 Proteomic Analyzer is a mass spectrometry instrument designed for protein identification and quantification. It utilizes matrix-assisted laser desorption/ionization (MALDI) technology to analyze protein samples.
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Lab products found in correlation
2 protocols using abi 4800 proteomic analyzer
1
Peptide Mass Fingerprinting for Protein Identification
2
Wheat Protein Identification by MALDI-TOF/TOF-MS
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The DAP spots from 2-DE gels were excised and digested with trypsin in 2 mL centrifuge tubes as described by Lv et al. [92 (link)] After bleaching and drying, the tryptic peptides analysis was performed by ABI 4800 Proteomic Analyzer matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (MALDI-TOF/TOF-MS) instrument (Applied Biosystems/MDS Sciex, USA). The MS/MS spectra were searched against the NCBI wheat protein database (136,744 entries)-the nonredundant Ensembl plants database of 2021 using software MASCOT version 2.1 (Matrix Science), and the related parameter settings were according to Cao et al. [90 (link)].
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