Anti zo 1

Manufactured by Beyotime

Sourced in China

The Anti-Zo-1 is a laboratory equipment used to detect and quantify the expression of the Zonula Occludens-1 (Zo-1) protein, which is a key component of tight junctions in epithelial and endothelial cells. The product is designed to facilitate research and analysis related to cell-cell adhesion, barrier function, and related cellular processes.

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Lab products found in correlation

Goat anti rabbit igg secondary antibody, by Abcam (1 mentions) Fluorescence microscope, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Actin tracker green 488, by Beyotime (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) Protease and phosphatase inhibitor, by Beyotime (1 mentions) Anti nrf2, by Abcam (1 mentions) Anti ho 1, by Abcam (1 mentions) Anti keap1, by Wuhan Servicebio Technology (1 mentions) Anti p nf κb, by Abcam (1 mentions) Anti nf κb, by Abcam (1 mentions)

2 protocols using anti zo 1

Immunofluorescent Localization of Tight Junction Protein Zo-1

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After fixation in 4% paraformaldehyde, permeabilization in 0.1% Triton X-100 and blockade with 1% bull serum albumin, the cells were incubated with anti-Zo-1 (Beyotime, Beijing, China) at 4 °C overnight and then goat anti-rabbit IgG secondary antibody (Abcam, Cambridge, UK) and Actin-Tracker-Green-488 (Beyotime, Beijing, China) for 1 h at room temperature. After removing the secondary antibodies, DAPI (Beyotime, Beijing, China) was applied to the cells. Images were taken with a fluorescence microscope (Olympus, Hamburg, Germany).

Zhang Y., Zhou Z., Xiao W., Tang Y., Guan W., Wang J., Shu F., Shen J., Gu S., Zhang L., Wang Q, & Xie L. (2022). Inosine and D-Mannose Secreted by Drug-Resistant Klebsiella pneumoniae Affect Viability of Lung Epithelial Cells. Molecules, 27(9), 2994.

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Intestinal Protein Expression Analysis

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Jejunum mucosa samples (0.1 g) were homogenized in RIPA lysis buffer plus protease and phosphatase inhibitors (Beyotime Biotechnology, Shanghai China). The western blot protocol was described in our previous report (Liu et al., 2022 ). Primary antibodies included: anti-β-actin (Cat. AF5003, Beyotime), anti-ZO-1 (Cat. GB111402, Servicebio), anti-Keap1 (Cat. GB111402, Servicebio), anti-Nrf2 (Cat. ab31163, Abcam), anti-HO-1 (Cat. bs-2075R, Bioss), anti-NF-κB (Abcam), and anti-p-NF-κB (Abcam). A goat anti-rabbit IgG-HRP antibody was used as secondary antibody. Relative protein abundance was quantified from 3 blots using Image-J software (Bethesda, MD).

Liu H., Meng H., Du M., Lv H., Wang Y, & Zhang K. (2024). Chlorogenic acid ameliorates intestinal inflammation by inhibiting NF-κB and endoplasmic reticulum stress in lipopolysaccharide-challenged broilers. Poultry Science, 103(5), 103586.

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