293T (CRL-3216) and Jurkat T cells (E6.1) were obtained from ATCC (American Type Culture Collection). They were routinely checked for mycoplasma and were mycoplasma free. transduced with pLenti-TOPO containing wild-type CD28 and the CD28 mutants. CD28 expression was detected with a mouse anti-human CD28 (Clone 28.2; dilution 1:100; eBioscience). The binding of human CD80-Fc (catalog number 140-B1-100; R&D Systems) and human CD86-Fc (catalog number 141-B2-100; R&D Systems) to these cells were performed as previously described29 (link),58 (link). The secondary antibody used was Alexa Fluor 647 conjugated goat anti-human IgG secondary antibody (catalog number A-21445; concentration: 5 μg/ml; Life Technologies). Kd were calculated based on the presumption of one binding site. Kd= [A][B]/[AB] where in A represent soluble CD80-Fc or Cd86-Fc and B represents CD28 molecules on the cell surface of 293T cells.
Mouse anti human cd28 clone 28.2
Manufactured by Thermo Fisher Scientific
Mouse anti-human CD28 (Clone 28.2) is a monoclonal antibody that binds to the CD28 receptor on human cells. CD28 is a co-stimulatory molecule that plays a crucial role in T cell activation and function.
Automatically generated - may contain errors
Lab products found in correlation
Human cd80 fc, by R&D Systems (2 mentions)
Human cd86 fc, by R&D Systems (2 mentions)
Alexa fluor 647 conjugated goat anti human igg secondary antibody, by Thermo Fisher Scientific (2 mentions)
293t crl 3216, by American Type Culture Collection (2 mentions)
Jurkat e6.1 t cells, by American Type Culture Collection (2 mentions)
2 protocols using mouse anti human cd28 clone 28.2
1
CD28 Receptor Binding Kinetics
2
CD28 Receptor Binding Kinetics
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293T (CRL-3216) and Jurkat T cells (E6.1) were obtained from ATCC (American Type Culture Collection). They were routinely checked for mycoplasma and were mycoplasma free. transduced with pLenti-TOPO containing wild-type CD28 and the CD28 mutants. CD28 expression was detected with a mouse anti-human CD28 (Clone 28.2; dilution 1:100; eBioscience). The binding of human CD80-Fc (catalog number 140-B1-100; R&D Systems) and human CD86-Fc (catalog number 141-B2-100; R&D Systems) to these cells were performed as previously described29 (link),58 (link). The secondary antibody used was Alexa Fluor 647 conjugated goat anti-human IgG secondary antibody (catalog number A-21445; concentration: 5 μg/ml; Life Technologies). Kd were calculated based on the presumption of one binding site. Kd= [A][B]/[AB] where in A represent soluble CD80-Fc or Cd86-Fc and B represents CD28 molecules on the cell surface of 293T cells.
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