De-identified peripheral blood samples (Leukopaks) were obtained from healthy adult male donors at the Gulf Coast Regional Blood Center (Houston, TX, USA). The work was conducted with approval from Rice University’s IRB. The anticoagulated blood was obtained at room temperature, no longer than 24 hours after draw, and immediately processed for isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation using Ficoll-Paque Premium 1.077 g/mL (GE Healthcare, IL; 17544202) according to the manufacturer’s instructions. PBMCs were either processed for sorting of monocytes or frozen (5 x 107 cells/mL) in complete RPMI with 5% (v/v) dimethyl sulfoxide. Monocytes were sorted from PBMCs via magnetically-activated cell sorting (MACS) through negative selection, using the Pan Monocyte Isolation Kit (Miltenyi Biotec, Germany; 130-096-537) and autoMACS Separator Pro or MiniMACS separator (Miltenyi Biotec), following manufacturer’s instructions.
Ficoll paque premium 1.077 g ml
Manufactured by GE Healthcare
Sourced in United States
Ficoll-Paque Premium 1.077 g/mL is a sterile, pyrogen-tested density gradient medium used for the isolation of mononuclear cells from whole blood or bone marrow.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by American Type Culture Collection (2 mentions)
Unlabeled oxldl, by Biomedical Technologies (2 mentions)
Pan monocyte isolation kit, by Miltenyi Biotec (1 mentions)
Penicillin streptomycin, by Lonza (1 mentions)
Percoll, by GE Healthcare (1 mentions)
Macrophage colony stimulating factor m csf, by Thermo Fisher Scientific (1 mentions)
Human serum, by MP Biomedicals (1 mentions)
Human buffy coats, by GE Healthcare (1 mentions)
Alamarblue assay, by Thermo Fisher Scientific (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Ptfe syringe filter, by Thermo Fisher Scientific (1 mentions)
Macrophage colony stimulating factor, by Thermo Fisher Scientific (1 mentions)
Polytetrafluoroethylene, by Thermo Fisher Scientific (1 mentions)
3 protocols using ficoll paque premium 1.077 g ml
1
Isolation and Purification of Monocytes from Peripheral Blood
2
Synthesis and Characterization of Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and materials were purchased from Sigma-Aldrich (Milwaukee, WI, USA) and were used as received unless noted otherwise. Hydrochloric acid (HCl, 1 N) was purchased from Fisher Scientific (Fair Lawn, NJ, USA), and silica from VWR (Radnor, PA, USA). The confined impinging jet mixer was provided by Prof. Robert Prud’homme at Princeton University (Princeton, NJ, USA). Cell culture assays used human buffy coats purchased from the New York Blood Center (New York, NY, USA) or New Jersey Blood Center (East Orange, NJ, USA), Ficoll-Paque premium 1.077 g/mL and Percoll 1.3 g/mL from GE Healthcare (Fairfield, CT, USA), FEP Teflon cell culture bags from Cellgenix, RPMI-1640 from ATCC (Manassas, VA, USA), macrophage colony stimulating factor (MCSF) from PeproTech (Rocky Hill, NJ, USA), penicillin/streptomycin from Lonza (Basel, Switzerland), fetal bovine serum (FBS) from Life Technologies (Carlsbad, CA, USA), human serum from MP Biomedical, unlabeled oxLDL from Biomedical Technologies Inc. (Ward Hill, MA, USA), and 3,3′-dioctadecyloxacarbocyanine (DiO) labeled oxLDL from Kalen Biomedical (Montgomery Village, MD, USA).
3
Optimized Macrophage Foam Cell Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and solvents
were purchased from Sigma-Aldrich (Milwaukee, WI) and used as received
unless otherwise noted. Hydrochloric acid (HCl, 1 N), dibenzyl tartrate
(DBT), and polytetrafluoroethylene (PTFE) syringe filters were purchased
from Fisher Scientific (Fair Lawn, NJ). Silica gel was purchased from
VWR (Radnor, PA). Monomethoxy-poly(ethylene glycol)-amine (mPEG-amine)
was purchased from Laysan Bio (Arab, AL) and azeotropically distilled
with toluene prior to use. Reagents for cell culture, toxicity studies,
and oxLDL uptake studies include human buffy coats purchased from
The Blood Center of New Jersey (East Orange, NJ), Ficoll-Paque premium
1.077 g/mL purchased from GE Healthcare (Fairfield, CT), RPMI-1640
purchased from ATCC (Manassas, VA), macrophage colony stimulating
factor purchased from PeproTech (Rocky Hill, NJ), penicillin/streptomycin
purchased from Lonza (Basel, Switzerland), alamarBlue assay, fetal
bovine serum, and Hoechst 33342 purchased from Life Technologies (Carlsbad,
CA), unlabeled oxLDL purchased from Biomedical Technologies Inc. (Ward
Hill, MA), and 3,3′-dioctadecyloxacarbocyanine (DiO) labeled
oxLDL purchased from Kalen Biomedical (Montgomery Village, MD).
were purchased from Sigma-Aldrich (Milwaukee, WI) and used as received
unless otherwise noted. Hydrochloric acid (HCl, 1 N), dibenzyl tartrate
(DBT), and polytetrafluoroethylene (PTFE) syringe filters were purchased
from Fisher Scientific (Fair Lawn, NJ). Silica gel was purchased from
VWR (Radnor, PA). Monomethoxy-poly(ethylene glycol)-amine (mPEG-amine)
was purchased from Laysan Bio (Arab, AL) and azeotropically distilled
with toluene prior to use. Reagents for cell culture, toxicity studies,
and oxLDL uptake studies include human buffy coats purchased from
The Blood Center of New Jersey (East Orange, NJ), Ficoll-Paque premium
1.077 g/mL purchased from GE Healthcare (Fairfield, CT), RPMI-1640
purchased from ATCC (Manassas, VA), macrophage colony stimulating
factor purchased from PeproTech (Rocky Hill, NJ), penicillin/streptomycin
purchased from Lonza (Basel, Switzerland), alamarBlue assay, fetal
bovine serum, and Hoechst 33342 purchased from Life Technologies (Carlsbad,
CA), unlabeled oxLDL purchased from Biomedical Technologies Inc. (Ward
Hill, MA), and 3,3′-dioctadecyloxacarbocyanine (DiO) labeled
oxLDL purchased from Kalen Biomedical (Montgomery Village, MD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!