A Beckman Coulter P/ACE MDQ CE instrument was utilized; data was collected at a voltage of 9.0 kV for 55 min with the UV detector set to 254 nm. The cartridge and sample storage temperatures were 30.0 and 25 °C, respectively.
P ace mdq ce instrument
Manufactured by Beckman Coulter
Sourced in United States
The P/ACE MDQ CE instrument is a capillary electrophoresis system designed for the separation and analysis of a wide range of biomolecules, including proteins, peptides, and nucleic acids. The instrument utilizes an automated, high-performance capillary electrophoresis platform to provide precise and reproducible results.
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Lab products found in correlation
2 protocols using p ace mdq ce instrument
1
Capillary Electrophoresis Analysis
2
Carbohydrate Analysis by Capillary Electrophoresis
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Carbohydrates (glucose, fructose and sucrose) were analyzed by capillary electrophoresis (CE) modified from a method of Rovio et al. [25] (link), with a P/ACE MDQ CE instrument (Beckman-Coulter, Fullerton, CA, USA) using diode array detection at 270 nm. Uncoated fused-silica capillaries of I.D. 25 µm and length 30/40 cm (effective/total length) were used. The ethyl acetate honey extracts were injected at a pressure of 0.5 psi for 10 s. The separation voltage was raised linearly within a 1 min ramp time from 0 to +16 kV. Calibration curves for the quantification of glucose, fructose and sucrose were prepared using standard solutions with concentrations between 10 and 430 mg/L.
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