Indocyanine green (ICG), doxorubicin hydrochloride (DOX ·HCl) and bovine serum albumin (BSA) [R-TE-156, CAS:3599–32-4; R-H-65212, CAS:25316–40-9; R-FB-001, Rui xi Biological Co. Ltd. Xi’an, China]. Ethanol (anhydrous) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich. 4′,6-diamidino-2-phenylindole (DAPI) (C0065, Solarbio Science & Technology Co., Ltd. Beijing, China). Dulbecco’s modified eagle medium (DMEM) cell culture medium and 1 × Phosphate Buffered Saline (PBS) buffer (PH=7.4) (B540626-0500, Sangon Biotech Co., Ltd. Shanghai, China). Deionized (DI) water was collected from a Milli-Q water purification system (Type 1, Millipore China Co., Ltd, New Jersey state, USA). All other chemicals and reagents were of analytical grade as purchased.
Phosphate buffered saline buffer
Manufactured by Sangon
Sourced in China
Phosphate Buffered Saline (PBS) is a commonly used buffer solution in various laboratory applications. It is a balanced salt solution that maintains a stable pH, typically around 7.4. PBS is primarily used to maintain the physiological conditions of cells and tissues, as well as to dilute and wash biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Polyethylene glycol peg 6000, by Merck Group (1 mentions)
Sodium acetate, by Beijing Chemical Works (1 mentions)
Absolute ethyl alcohol, by Beijing Chemical Works (1 mentions)
Tris hcl buffer, by Beyotime (1 mentions)
Proteinase k, by Beyotime (1 mentions)
4 protocols using phosphate buffered saline buffer
1
Nanoscale Drug Delivery System Formulation
2
Synthesis and Characterization of Iron-Citrate Nanoparticles
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Ferric chloride hexahydrate (FeCl3·6H2O) and trisodium citrate dihydrate were purchased from Tianjin Damao Chemical Institute (Tianjin China). Sodium acetate and absolute ethyl alcohol were obtained from Beijing Chemical Reagent Company (Beijing, China). Poly(ethylene glycol) (PEG6000) and N-Hydroxysuccinimide (NHS) were bought from Sigma Company (USA). N-(3-dimethylamlnopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) was bought from Aladdin Chemistry Co. Ltd. (Shanghai, China). Bovine serum albumin (BSA) was purchased from Celartics biopharma Co. Ltd. 2-Morpholinoethanesulfonic acid (MES) was purchased from TCI Ltd. (Shanghai, China). All the above reagents were of analytical grade.
Sephacryl s-100 High Resolution was purchased by GE Healthcare Institute (Sweden). The phosphate-buffered saline (PBS) buffer was bought from Sangon Biotech (Shanghai, China) Co., Ltd. The BCA protein content kit was purchased from DingGuo Company (Beijing, China). The TIANamp Bacteria DNA Kit was obtained from Tiangen Bio (Beijing, China). Deionized water and S. aureus were from the School of Public Health, Jilin University.
Sephacryl s-100 High Resolution was purchased by GE Healthcare Institute (Sweden). The phosphate-buffered saline (PBS) buffer was bought from Sangon Biotech (Shanghai, China) Co., Ltd. The BCA protein content kit was purchased from DingGuo Company (Beijing, China). The TIANamp Bacteria DNA Kit was obtained from Tiangen Bio (Beijing, China). Deionized water and S. aureus were from the School of Public Health, Jilin University.
3
Sensitive Detection of Alzheimer's Biomarker
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All oligonucleotides used in this work were synthesized by Sangon Biotech Co., Ltd. Detailed sequences are listed in the SI Appendix . Phosphate-buffered saline (PBS) buffer, DEPC-treated water (diethyl pyrocarbonate), and Tris-EDTA buffer were purchased from Sangon Biotech Co., Ltd. The streptavidin-functionalized MNP, Tris-(2-carboxyethyl)-phosphine hydrochloride (TCEP), and sodium dodecyl sulfate (SDS) were purchased from Aladdin. Proteinase K and Tris ⋅ HCl buffer were purchased from Beyotime Biotechnology. Aβ1–42 peptide and monoclonal antibodies (mAbs) of Aβ1–42 (clone 12F4) specific to the C-termini and a mAb capable of binding to the N terminus of Aβ1–42 peptide (clone 6E10) were obtained from BioLegend, Inc. The streptavidin-functionalized AuNPs were purchased from Nanopartz. Thiol-Poly(ethylene glycol) (PEG)-hydroxyl (molecular weight: 500 Da) Thiol-PEG-biotin (molecular weight: 3,400 Da), and streptavidin were purchased from Sigma-Aldrich. All solutions were prepared with deionized water (18.2 MΩ ⋅ cm−1) from a Millipore system. All experiments were repeated in triplicates unless mentioned otherwise.
4
Quantification of Fly Muscle MDA
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Twenty fly muscles were removed from the thorax and placed in 200 μL of Phosphate Buffered Saline (PBS) buffer (Sangon, Shanghai, China). After that, the sample is stored at -20 degrees Celsius and reserved. For the formal test, flies muscle were converted to homogenates in a homogenizer filled with 1 mL PBS (pH 7.2-7.4). The homogenates were centrifuged at 4 °C for 15 min with a speed of 2000 r/min. The supernatant was mixed with the reagents supplied in an MDA Assay Kit (MLBIO, Shanghai, China) and incubated at 95 °C for 40 min. The absorbance of the supernatant was measured at 530 nm. All operations were according to the manufacturer's instructions. All assays were repeated three times.
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