Formalin-fixed paraffin–embedded (FFPE) tumor samples from 53 of the 89 (60%) patient pre-treatment biopsies were adequate for tissue microarray (TMA) construction. After pathologist review (AM) to confirm squamous cell histology and the location of tumor for sampling, TMAs were assembled from triplicate 0.6 mm cores of FFPE primary tumor samples using a Beecher Manual Tissue Arrayer (Beecher Instruments, Sun Prairie, WI, USA).
Beecher manual tissue arrayer
Manufactured by Beecher Instruments
Sourced in United States
The Beecher Manual Tissue Arrayer is a laboratory instrument designed for the construction of tissue microarrays. It allows for the precise extraction and transfer of small tissue samples from donor tissue blocks to recipient paraffin blocks, enabling parallel analysis of multiple tissue samples on a single slide.
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3 protocols using beecher manual tissue arrayer
1
Tissue Microarray Construction from FFPE Tumor Samples
2
Tissue Microarray Construction for IHC Analysis
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Formalin-fixed and paraffin-embedded tumor specimens were prepared for TMA using the Beecher Manual Tissue Arrayer (Beecher Instruments, Inc., Sun Prairie, WI, USA). Briefly, one core tissue biopsy with a diameter of 1 mm was taken from a representative region of an individual paraffin-embedded BC sample and placed into a new recipient paraffin block. Every sample included 2–3 tissue cores for biomarker analysis. Consecutive sections of 4–5 mm were cut from TMA blocks and placed on glass slides for subsequent immunohistochemical analysis. The tumor blocks also contained tumor and normal breast tissue samples as positive and negative controls for each IHC staining.
3
Tissue Microarray Construction and Analysis
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Tissue cores with a diameter of 0.6 mm were isolated from paraffin-embedded formalin-fixed tissue and transferred into a recipient paraffin block by using the Beecher Manual Tissue Arrayer (Beecher Instruments, Inc., Sun Prairie, WI, USA). The selected tissue cores were chosen by an experienced pathologist from our hospital based on H&E staining of tissue sections. Then, consecutive sections of 4–5 μm were cut from TMA blocks and placed on glass slides for subsequent immunohistochemical analysis.
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