Rosetta competent cells

Manufactured by Merck Group

Rosetta Competent Cells are a specialized laboratory tool developed by Merck Group. They are designed to assist in the expression of recombinant proteins from organisms that utilize rare codons. The cells provide tRNAs to facilitate the translation of these rare codons, enabling the efficient production of proteins that may otherwise be challenging to express.

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Lab products found in correlation

Pgex 6p 1 expression vector, by GE Healthcare (2 mentions)

Close spelling variants of this product

Rosetta 2(DE3) competent E. coli cells Rosetta™ (DE3) competent cells Rosetta 2(DE3) Competent Cells Rosetta 2(DE3)pLysS Singles Competent Cells E. coli Rosetta 2(DE3) Singles Competent Cells Rosetta™(DE3)pLysS Competent Cells Rosetta 2(DE3) pLysS competent cells

3 protocols using rosetta competent cells

Purification of RBFOX2 RRM Domain

Cited 3 times

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The RRM domain of RBFOX2 (amino acids 100–194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28–9546-48) downstream of a GST-SBP tandem affinity tag. Following 12 hr recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12°C with ampicillin and chloramphenicol selection, the protein was expressed by addition of IPTG and purified via the GST tag as described previously^{26 (link),28 (link),38 (link)}.

Begg B.E., Jens M., Wang P.Y., Minor C.M, & Burge C.B. (2020). Concentration-dependent splicing is enabled by Rbfox motifs of intermediate affinity. Nature structural & molecular biology, 27(10), 901-912.

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Purification of RBFOX2 RRM Domain

Cited 3 times

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Rosetta Expression of Eukaryotic Proteins

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Plasmid constructs were transformed into Rosetta competent cells (Millipore, Billerica, MA), which are well suited for eukaryotic protein expression. One-liter cultures were grown in LB using the appropriate selective antibiotics: chloramphenicol (34 μg/mL) for the pRARE plasmid in the Rosetta strain, and ampicillin (100 μg/mL) for the pET vectors. Cultures were grown at 37° C with 225 rpm shaking until 0.55 OD600, and then protein expression was induced with 1 mM IPTG. Expression occurred overnight at 17°
C with shaking at 200 rpm. Cells were harvested by centrifugation at 5500 x g for 15 minutes at 4 °C and the pellets stored at -20 °C until further use.

Bradford A.M., Koirala R., Park C.K, & Lyons B.A. (2019). Characterization of the full-length human Grb7 protein and a phosphorylation representative mutant. Journal of molecular recognition : JMR, 32(11).

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