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Facs scan flow cytometry

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FACS scan flow cytometry is a laboratory instrument used for analyzing and sorting cells or particles in a fluid sample. It measures multiple physical characteristics of individual particles, such as their size and internal complexity, as the particles pass through a laser beam.

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Lab products found in correlation

Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by BD (1 mentions) Fluo 3 am, by BD (1 mentions) Fluo 3 am, by Beyotime (1 mentions) Cellquest software, by BD (1 mentions) Propidium iodide, by BD (1 mentions)

Close spelling variants of this product

Flow cytometry (2933 citations) FACS flow cytometry (64 citations) FACS cytometry (22 citations) BD FACSTM (2 citations)

4 protocols using facs scan flow cytometry

1

Annexin V-FITC Apoptosis Assay

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An annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit (Becton-Dickinson, CA, USA) was used to assess apoptosis. After experimental treatments, ARPE-19 cells were collected and suspended in 1× binding buffer containing annexin V-FITC and propidium iodide (PI) according to the manufacturer instructions. Fluorescence was measured with a FACS scan flow cytometry (Becton-Dickinson, San Jose, CA, USA).
Zhou X., Wei Y., Qiu S., Xu Y., Zhang T, & Zhang S. (2016). Propofol Decreases Endoplasmic Reticulum Stress–Mediated Apoptosis in Retinal Pigment Epithelial Cells. PLoS ONE, 11(6), e0157590.
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2

Measuring Intracellular Calcium Levels

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The cell-permeable calcium-sensitive fluorescent dye Fluo-3/AM (Beyotime, Haimen, China) was used to test the free cytosolic calcium levels. Cells were washed with PBS, and then were incubated with 5 μM Fluo-3/AM at 37°C for 1 h in the dark after the treatment with TG and propofol for 12 h. Then cells were washed with PBS for 3 times and fluorescence of Fluo-3 combined with cytosolic calcium was analyzed by FACS scan flow cytometry (Becton-Dickinson, San Jose, CA, USA).
Zhou X., Wei Y., Qiu S., Xu Y., Zhang T, & Zhang S. (2016). Propofol Decreases Endoplasmic Reticulum Stress–Mediated Apoptosis in Retinal Pigment Epithelial Cells. PLoS ONE, 11(6), e0157590.
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3

Monitoring MMP Alteration by Flow Cytometry

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The alteration of MMP was monitored by flow cytometry as previously described by Chen et al (30 (link)). The cells were harvested and washed twice with PBS. All samples were stained with DiOC6 (40 nM) for 30 min at 37°C and the fluorescence of DiOC6 was analyzed by FACSscan flow cytometry (BD Biosciences), using CellQuest software (version 5.1; BD Biosciences).
Huang C.F., Liu S.H., Ho T.J., Lee K.I., Fang K.M., Lo W.C., Liu J.M., Wu C.C, & Su C.C. (2022). Quercetin induces tongue squamous cell carcinoma cell apoptosis via the JNK activation-regulated ERK/GSK-3α/β-mediated mitochondria-dependent apoptotic signaling pathway. Oncology Letters, 23(3), 78.
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4

Cell Cycle Analysis by BrdU and DNA Content

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Cell cycle was analyzed by determining bromodeoxyuridine incorporation compared with DNA content. A total of 3 × 105 cells were initially plated into a 60-mm plate. The following day, drugs at indicated concentrations were added into the cell medium. After the indicated time, cells were processed according to the manufacturer’s protocol (BD Biosciences). Cell cycle was also analyzed by DNA content. Cells with or without treatment were trypsinized and fixed with 70% ethanol at −20°C overnight. DNA was labeled with propidium iodide (BD Bioscience) at 37°C for 15 minutes. Approximately 10,000 cells were collected for each assay and analyzed using FACS Scan flow cytometry (BD Biosciences).
Galan-Cobo A., Sitthideatphaiboon P., Qu X., Poteete A., Pisegna M.A., Tong P., Chen P.H., Boroughs L.K., Rodriguez M.L., Zhang W., Parlati F., Wang J., Gandhi V., Skoulidis F., DeBerardinis R.J., Minna J.D, & Heymach J.V. (2019). LKB1 and KEAP1/NRF2 pathways cooperatively promote metabolic reprogramming with enhanced glutamine dependence in KRAS-mutant lung adenocarcinoma. Cancer research, 79(13), 3251-3267.
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